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Saccharomonospora oceani sp. nov. isolated from marine sediments in Little Andaman, India

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Abstract

Two actinomycete strains, designated YIM M11168T and YIM M11177, were isolated from marine sediment samples from Little Andaman, Indian Ocean, and their taxonomic position was determined by a polyphasic approach. The two Gram-positive, aerobic strains were observed to produce branched substrate mycelium and aerial hyphae but did not fragment, and no diffusible pigment was produced on the media tested. At maturity, spores were formed singly or in pairs on aerial hyphae and substrate mycelium, and occasionally the single ones were borne on long sporophores. The optimum growth was determined to occur at 28 °C, 0–4 % (w/v) NaCl and pH 7.0–8.0. Whole-cell hydrolysates of both strains contained meso-diaminopimelic acid and the diagnostic sugars were determined to be galactose, glucose and arabinose. Their predominant menaquinone was found to be MK-9(H4). The polar lipids detected in the two strains were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, phosphatidylmethylethanolamine, phosphatidylethanolamine and two unknown phosphoglycolipids. The major fatty acids (>10 %) identified were iso-C16:0, iso-C16:1 H, iso-C16:0, C17:1 ω6c for strain YIM M11168T, iso-C16:0 and Summed Feature 3 for strain YIM M11177. The G + C contents of the genomic DNAs of both strains were determined to be 71.4 %. DNA–DNA hybridization relatedness values (78.4 ± 3.7 %) of these two isolates supported the conclusion that they belong to the same species. Based on phylogenetic analysis, phenotypic and genotypic data, it is concluded that the two isolates belong to a novel species of the genus Saccharomonospora of the family Pseudonocardiaceae. The name Saccharomonospora oceani sp. nov. (Type strain YIM M11168T = DSM 45700T = JCM 18128T) is proposed for the novel species.

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Acknowledgments

The authors are grateful to Prof. Yao-Jian Huang for his kind providing the type strain of S. marina XMU15T and Prof. Jean P Euzéby’s help with the Latin etymology for the new species. This research was supported by the National Basic Research Program of China (No. 2010CB833801). W-J Li was also supported by ‘Hundred Talents Program’ of the Chinese Academy of Sciences.

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Authors and Affiliations

  1. Key Laboratory of Biogeography and Bioresource in Arid Land, CAS, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi, 830011, People’s Republic of China

    Dao-Feng Zhang & Wen-Jun Li

  2. Hongyun Honghe Tobacco (Group) Co., Ltd, Kunming, 650202, People’s Republic of China

    Wei Chen

  3. C.A.S in Marine Biology, Faculty of Marine Science, Annamalai University, Parangipettai, 608 502, Tamilnadu, India

    Maloy Kumar Sahu & Kannan Sivakumar

  4. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Dao-Feng Zhang, Jie He, Xiao-Mei Zhang, Zi-jun Xiong & Wen-Jun Li

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  1. Dao-Feng Zhang
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  2. Wei Chen
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  3. Jie He
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Correspondence to Wen-Jun Li.

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Dao-Feng Zhang and Wei Chen contributed equally to this study.

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Zhang, DF., Chen, W., He, J. et al. Saccharomonospora oceani sp. nov. isolated from marine sediments in Little Andaman, India. Antonie van Leeuwenhoek 103, 1377–1384 (2013). https://doi.org/10.1007/s10482-013-9918-0

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