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Sphingomonas ginsenosidivorax sp. nov., with the ability to transform ginsenosides

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Abstract

A Gram-negative, strictly aerobic, non-motile, non-spore-forming and rod-shaped bacterial strain designated KHI67T was isolated from sediment of the Gapcheon River in South Korea and its taxonomic position was investigated by using a polyphasic approach. Strain KHI67T was observed to grow optimally at 25–30 °C and at pH 7.0 on nutrient and R2A agar. On the basis of 16S rRNA gene sequence similarity, strain KHI67T was shown to belong to the family Sphingomonadaceae and was related to Sphingomonas faeni MA-olkiT (97.6 % sequence similarity), Sphingomonas aerolata NW12T (97.5 %) and Sphingomonas aurantiaca MA101bT (97.3 %). The G + C content of the genomic DNA was determined to be 65.6 %. The major ubiquinone was found to be Q-10, the major polyamine was identified as homospermidine and the major fatty acids identified were summed feature 8 (comprising C18:1 ω7c/ω6c; 37.0 %), C16:0 (13.0 %), summed feature 3 (comprising C16:1 ω7c/C16:1 ω6c; 12.8 %) and C14:0 2OH (9.3 %). DNA and chemotaxonomic data supported the affiliation of strain KHI67T to the genus Sphingomonas. The DNA–DNA relatedness values between strain KHI67T and its closest phylogenetic neighbours were below 15 %. Strain KHI67T could be differentiated genotypically and phenotypically from the recognised species of the genus Sphingomonas. The isolate therefore represents a novel species, for which the name Sphingomonas ginsenosidivorax sp. nov. is proposed, with the type strain KHI67T (=KACC 14951T = JCM 17076T = LMG 25801T).

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Acknowledgments

This work was supported by the Intelligent Synthetic Biology Center of Global Frontier Project funded by the Ministry of Education, Science and Technology (2011-0031967) and by the project on survey and excavation of Korean indigenous species of the National Institute of Biological Resources (NIBR) under the Ministry of Environment, Korea.

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Authors and Affiliations

  1. KI for the Biocentury, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701, Republic of Korea

    Xue-Feng Jin, Jin-Kwang Kim, Qing-Mei Liu, Dan He, Sun-Chang Kim & Wan-Taek Im

  2. Intelligent Synthetic Biology Center, 373-1, Guseong-dong, Yuseong-gu, Daejeon, 305-701, Republic of Korea

    Qing-Mei Liu & Sun-Chang Kim

  3. Microorganism Resources Division, National Institute of Biological Resources, Incheon, 404-708, Republic of Korea

    Myung-Suk Kang

  4. College of Biotechnology, Dalian Polytechnic University, Qinggong-yuan No. 1, Ganjingzi-qu, Dalian, 116034, People’s Republic of China

    Dan He & Feng-Xie Jin

  5. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701, Republic of Korea

    Sun-Chang Kim

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  1. Xue-Feng Jin
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Correspondence to Wan-Taek Im.

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Jin, XF., Kim, JK., Liu, QM. et al. Sphingomonas ginsenosidivorax sp. nov., with the ability to transform ginsenosides. Antonie van Leeuwenhoek 103, 1359–1367 (2013). https://doi.org/10.1007/s10482-013-9916-2

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