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Bacillus enclensis sp. nov., isolated from sediment sample

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Abstract

A novel bacterial strain, designated SGD-1123T was isolated from Chorao Island, in Goa Province, India. The strain was found to be able to grow at 15–42 °C, pH 5–12 and 0–12 % (w/v) NaCl. The whole cell hydrolysates were found to contain meso-diaminopimelic acid, galactose and arabinose. The major fatty acids were identified as iso-C15:0 and anteiso-C15:0, MK-7 was identified as the predominant menaquinone and the predominant polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified aminolipid. The genomic DNA G+C content was determined to be 44.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences placed the isolate within the genus Bacillus and further revealed that strain SGD-1123T had highest sequence similarity with Bacillus aquimaris, and forms a separate clade with its closest relatives i.e. B. aquimaris, Bacillus vietnamensis and Bacillus marisflavi, with which it shares 94.5, 94.1 and 94.1 % similarity respectively. The phylogenetic, chemotaxonomic and phenotypic analyses indicated that strain SGD-1123T represents a novel species within the genus Bacillus, for which the name Bacillus enclensis is proposed. The type strain is SGD-1123T (NCIM 5450T=CCTCC AB 2011125T).

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Acknowledgments

SGD acknowledges the financial supports received under the Start up Grant Nos. MLP-027426 from the CSIR-National Chemical Laboratory, Pune, India.

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Authors and Affiliations

  1. NCIM-Resource Center, CSIR-National Chemical Laboratory, Pune, 411008, Maharashtra, India

    Syed G. Dastager & Rahul Mawlankar

  2. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, Yunnan, People’s Republic of China

    Shan-Kun Tang

  3. Microbial Type Culture Collection and Gene Bank (MTCC), CSIR-Institute of Microbial Technology, Sector-39A, Chandigarh, 160036, India

    Krishnamurthi Srinivasan

  4. Microbial Culture Collection (MCC), National Centre for Cell Science, Pune, 411007, Maharashtra, India

    V. Venkata Ramana & Yogesh S. Shouche

Authors
  1. Syed G. Dastager
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  2. Rahul Mawlankar
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  3. Shan-Kun Tang
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  4. Krishnamurthi Srinivasan
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  5. V. Venkata Ramana
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  6. Yogesh S. Shouche
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Correspondence to Syed G. Dastager.

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Dastager, S.G., Mawlankar, R., Tang, SK. et al. Bacillus enclensis sp. nov., isolated from sediment sample. Antonie van Leeuwenhoek 105, 199–206 (2014). https://doi.org/10.1007/s10482-013-0066-3

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  • DOI: https://doi.org/10.1007/s10482-013-0066-3

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