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Bacterial communities associated with the rhizosphere of pioneer plants (Bahia xylopoda and Viguiera linearis) growing on heavy metals-contaminated soils

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Abstract

In this study, the bacterial communities associated with the rhizospheres of pioneer plants Bahia xylopoda and Viguiera linearis were explored. These plants grow on silver mine tailings with high concentration of heavy metals in Zacatecas, Mexico. Metagenomic DNAs from rhizosphere and bulk soil were extracted to perform a denaturing gradient gel electrophoresis analysis (DGGE) and to construct 16S rRNA gene libraries. A moderate bacterial diversity and twelve major phylogenetic groups including Proteobacteria, Acidobacteria, Bacteroidetes, Gemmatimonadetes, Chloroflexi, Firmicutes, Verrucomicrobia, Nitrospirae and Actinobacteria phyla, and divisions TM7, OP10 and OD1 were recognized in the rhizospheres. Only 25.5% from the phylotypes were common in the rhizosphere libraries and the most abundant groups were members of the phyla Acidobacteria and Betaproteobacteria (Thiobacillus spp., Nitrosomonadaceae). The most abundant groups in bulk soil library were Acidobacteria and Actinobacteria, and no common phylotypes were shared with the rhizosphere libraries. Many of the clones detected were related with chemolithotrophic and sulfur-oxidizing bacteria, characteristic of an environment with a high concentration of heavy metal-sulfur complexes, and lacking carbon and organic energy sources.

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Acknowledgments

This work was supported by grants SIP20070651, SIP20070164, SIP20080688 and SIP20080628 Instituto Politécnico Nacional (IPN). Y. E. Navarro-Noya thanks the Consejo Nacional de Ciencia y Tecnología (CONACyT) and the Programa Institucional de Formación de Investigadores (PIFI), IPN, for scholarships. C. Hernández-Rodríguez and J. Jan-Roblero appreciate the COFAA, and EDI, IPN fellowships; and SNI, CONACyT. Our special thanks to L. M. Diaz-Garduño for her valuable assistance with the vegetal species identification.

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Authors and Affiliations

  1. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, IPN, Prol. de Carpio y Plan de Ayala s/n. Col. Sto. Tomás, 11340, Mexico, D.F., Mexico

    Yendi E. Navarro-Noya, Janet Jan-Roblero, Regina Hernández-Gama & César Hernández-Rodríguez

  2. Laboratorio de Microbiología Ambiental, Colegio de Postgraduados, Carr. Mexico-Texcoco Km 36.5 Montecillo, 56230, Edo. de Mexico, Mexico

    Maria del Carmen González-Chávez

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  1. Yendi E. Navarro-Noya
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  2. Janet Jan-Roblero
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  3. Maria del Carmen González-Chávez
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  4. Regina Hernández-Gama
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  5. César Hernández-Rodríguez
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Correspondence to César Hernández-Rodríguez.

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Navarro-Noya, Y.E., Jan-Roblero, J., del Carmen González-Chávez, M. et al. Bacterial communities associated with the rhizosphere of pioneer plants (Bahia xylopoda and Viguiera linearis) growing on heavy metals-contaminated soils. Antonie van Leeuwenhoek 97, 335–349 (2010). https://doi.org/10.1007/s10482-010-9413-9

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