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Selective position of individual cells without lysis on a circular window array using dielectrophoresis in a microfluidic device

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Abstract

We trapped individual cells between two circular windows using negative dielectrophoretic (DEP) force and then sequentially trapped them inside circular windows by positive DEP force without electrical lysis in a microfluidic device. Three parameters, (1) the transmembrane potential that determines the lysis of a cell, (2) individual cell size that affects the trapped position accuracy of the cell, and (3) the Clausius–Mossotti (CM) factor that decides the trapped efficiency of the cell, were characterized experimentally and numerically in this sequential cell trapping technique. In this characterization, we confirmed that the swap rate of applied voltage frequency, size similarity between the cell and circular window, and instantaneous change rate of Re(f CM) as a function of frequency were important factors in determining the selective position of individual cells without lysis. Our results provide useful suggestions for designing the structure of microfluidic DEP devices and optimizing variables required to manipulate individual cell trapping using both negative and positive DEP forces.

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Acknowledgements

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2013R1A2A2A03005767, NRF-2017R1A2B2002076), Republic of Korea, and by the Yonsei University Future-leading Research Initiative (2016-22-0065, 2015-22-0070).

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Author notes

  1. Young-Jin Jung and Taewoo Lee have contributed equally to this work.

Authors and Affiliations

  1. Department of Biomedical Engineering, Yonsei University, Wonju, 26493, Republic of Korea

    Young-Jin Jung, Seungyeop Choi, Sei-Young Lee, Jaehong Key, Yeong-Min Yoo, Han-Sung Kim & Sang Woo Lee

  2. Department of Biomedical Engineering, National University of Singapore, Singapore, 117583, Singapore

    Taewoo Lee

  3. Mechanical Engineering Department, University of Wisconsin-Milwaukee, Milwaukee, WI, 53211, USA

    Woo-Jin Chang

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  1. Young-Jin Jung
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  2. Taewoo Lee
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  3. Seungyeop Choi
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  9. Sang Woo Lee
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Corresponding authors

Correspondence to Han-Sung Kim or Sang Woo Lee.

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Jung, YJ., Lee, T., Choi, S. et al. Selective position of individual cells without lysis on a circular window array using dielectrophoresis in a microfluidic device. Microfluid Nanofluid 21, 150 (2017). https://doi.org/10.1007/s10404-017-1987-3

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  • DOI: https://doi.org/10.1007/s10404-017-1987-3

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