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Enantiomeric Separation of Drugs Using Ion Interaction Reagents in Combination with β-Cyclodextrin in HPLC

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Abstract

This work describes the use of β-cyclodextrin (β-CD) as a mobile phase additive in combination with ion interaction reagents (IIR) for the enantioresolution of cyclopentolate, 2,6-diketopiperazine derivative and methylphenobarbital. The effect of concentration and type of IIR and temperature on retention and enantioseparation were studied. It has been found that the addition of an IIR to β-CD solution can lead to the improvement in enantioresolution. Interestingly, the most significant enhancement of enantioresolution was achieved for cyclopentolate, most probably due to its highest basicity. Also the stability constants of the complexes formed between β-CD and model compounds in the presence of IIR were estimated. Significant increase of complexation rate of cyclopentolate with β-cyclodextrin in presence of IIRs was observed.

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Acknowledgments

The research was partially supported by the European Union within European Regional Development Fund, through grant Innovative Economy (POIG.01.01.02-14-102/09).

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Authors and Affiliations

  1. Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224, Warsaw, Poland

    Kamila Szwed & Monika Asztemborska

  2. Department of Drug Technology and Pharmaceutical Biotechnology, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097, Warsaw, Poland

    Maciej Dawidowski

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  1. Kamila Szwed
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  2. Maciej Dawidowski
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  3. Monika Asztemborska
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Correspondence to Kamila Szwed.

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Szwed, K., Dawidowski, M. & Asztemborska, M. Enantiomeric Separation of Drugs Using Ion Interaction Reagents in Combination with β-Cyclodextrin in HPLC. Chromatographia 76, 467–474 (2013). https://doi.org/10.1007/s10337-013-2416-8

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  • DOI: https://doi.org/10.1007/s10337-013-2416-8

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