Skip to main content
SpringerLink
Log in

Analysis of Neutral Sugars of Asparagus officinalis Linn. Polysaccharide by CZE with Amperometric Detection

  • Original
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

Analysis of the neutral sugars of Asparagus officinalis Linn. polysaccharide by different methods has yielded inconsistent results. In the work discussed in this paper, capillary zone electrophoresis with amperometric detection (CZE–AD) was used for analysis of neutral sugars in A. officinalis Linn. polysaccharide. The configuration of the wall-jet and the diameter of the copper disk electrode were investigated to achieve optimized detection sensitivity. The separation electrolyte, separation voltage, and injection time were studied for their effects on CZE separation. Under the optimum CZE–AD conditions, seven monosaccharides were separated to baseline by using 120 mM NaOH as separation electrolyte. Linear response was excellent and repeatability was satisfactory. It was found that Asparagus polysaccharide was composed of fucose, galactose, glucose, rhamnose, arabinose, fructose, and xylose at a mole ratio of 0.2:16.2:5.0:1.0:15.5:0.6:18.8. Compared with other methods, analysis of the composition of Asparagus polysaccharide by CZE–AD had the merits of rapidness, accuracy, and lower sampling volume.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Diwanay S, Chitre D, Patwardhan B (2004) J Ethnopharmacol 90:49–55. doi:10.1016/j.jep.2003.09.023

    Article  Google Scholar 

  2. Shao Y, Chin C, Ho C, Ma W, Garrison S, Huang M (1996) Cancer Lett 104:31–36. doi:10.1016/0304-3835(96)04233-4

    Article  CAS  Google Scholar 

  3. Ji Y, Ji C, Chen X (2008) IFMBE Proceedings 19:528–530

    Article  Google Scholar 

  4. Ji Y, Ji C, Chen X (2008) Chin Pharm J 43:1146–1149

    CAS  Google Scholar 

  5. Hopkins D, Chudek J, Bignell D, Frouz J, Webster E, Lawson T (1998) Biodegradation 9:423–431. doi:10.1023/A:1008313309557

    Article  CAS  Google Scholar 

  6. Wang Z, Fang J (2000) Chin J Anal Chem 28:240–247

    Google Scholar 

  7. Englyst H, Quigley M, Hudson G (1994) Analyst 119:1497–1509. doi:10.1039/AN9941901497

    Article  CAS  Google Scholar 

  8. Villanueva-Suarez M, Redondo-Cuenca A, Rodriaguez-Sevilla M, Martianez M (2003) J Agric Food Chem 51:5950–5955. doi:10.1021/jf021010h

    Article  CAS  Google Scholar 

  9. Grill E, Huber C, Oefner P, Vorndran A, Bonn G (1993) Electrophoresis 14:1004–1010. doi:10.1002/elps.11501401160

    Article  CAS  Google Scholar 

  10. Zhou W, Baldwin R (1996) Electrophoresis 17:319–324. doi:10.1002/elps.1150170206

    Article  CAS  Google Scholar 

  11. Guttman A, Chen F, Evangelista R (1996) Electrophoresis 17:412–417. doi:10.1002/elps.1150170221

    Article  CAS  Google Scholar 

  12. Ji Y, Chen X, Ji C, Chen B, Li M (2006) Chin Tradit Herbal Drugs 37:1159–1161

    CAS  Google Scholar 

  13. Yang Z, Li Z, Zhu J, Wang Q, He P, Fang Y (2010) J Sep Sci 33:1312–1318. doi:10.1002/jssc.200900586

    CAS  Google Scholar 

  14. Hu Q, Zhou T, Zhang L, Fang Y (2001) Analyst 126:298–301. doi:10.1039/B006223P

    Article  CAS  Google Scholar 

  15. Garcia C, Henry C (2005) Electroanalysis 17:1125–1130. doi:10.1002/elan.200403235

    Article  CAS  Google Scholar 

  16. Wang Q, Yu H, Zong J, He P, Fang Y (2003) J Pharm Biomed Anal 31:473–480. doi:10.1016/S0731-7085(02)00714-8

    Article  CAS  Google Scholar 

  17. Yang Z, Zhang L, Li G, Wang Q, He P, Fang Y (2010) Chromatographia 71:439–445. doi:10.1365/s10337-009-1459-3

    Article  CAS  Google Scholar 

  18. Fu C, Wang A, Song L, Fang Y (1998) Chin J Anal Chem 26:1085–1088

    CAS  Google Scholar 

  19. Lu W, Cassidy R (1993) Anal Chem 66:200–204. doi:10.1021/ac00074a004

    Article  Google Scholar 

  20. Slavik B (1986) Methods of studying plant water relations. Science Press, Beijing, p 129

    Google Scholar 

  21. Sevag M, Lackman D, Smolens J (1938) J Biol Chem 124:425–436

    CAS  Google Scholar 

  22. Lee H, Chen S (2004) Talanta 64:210–216. doi:10.1016/j.talanta.2004.02.013

    Article  CAS  Google Scholar 

  23. Colon L, Dadoo R, Zare R (1993) Anal Chem 65:476–481. doi:10.1021/ac00052a027

    Article  CAS  Google Scholar 

  24. Cahours X, Morin P, Dreux M (1999) J Chromatogr A 845:203–216. doi:10.1016/S0021-9673(99)00007-2

    Article  CAS  Google Scholar 

  25. Zhu S, Xiao Q, Chen H, Xu D, Li Z, Hua L (1998) J Instrumental Anal 17:9–12

    CAS  Google Scholar 

  26. Vorndran A, Oefner P, Scherz H, Bonn G (1992) Chromatographia 33:163–168. doi:10.1007/BF02275899

    Article  CAS  Google Scholar 

  27. Klockow A, Paulusa A, Figueiredo V, Amadò R, Widmer H (1994) J Chromatogr A 680:187–200. doi:10.1016/0021-9673(94)80067-7

    Article  CAS  Google Scholar 

  28. Li S (1992) Capillary Electrophoresis: Principle Practice and Application. Elsevier, The Netherlands, p 15

    Google Scholar 

  29. Ye J, Baldwin R (1993) Anal Chem 65:3525–3527. doi:10.1021/ac00071a034

    Article  CAS  Google Scholar 

  30. Waldron K, Selvemdran R (1990) Physiol Plantarum 80:568–575. doi:10.1111/j.1399-3054.1990.tb05680.x

    Article  CAS  Google Scholar 

Download references

Acknowledgment

This work was financially supported by Program for New Century Excellent Talents in University.

Author information

Authors and Affiliations

  1. Pharmacy College, Dali University, Wanhua Rd, Dali, Yunnan, 671000, People’s Republic of China

    Zhiyong Yang

  2. East China Normal University, North Zhongshan Rd, Shanghai, 200062, People’s Republic of China

    Huan Wang, Wen Zhang, Qingjiang Wang, Pingang He & Yuzhi Fang

Authors
  1. Zhiyong Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Huan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wen Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Qingjiang Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Pingang He
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yuzhi Fang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhiyong Yang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yang, Z., Wang, H., Zhang, W. et al. Analysis of Neutral Sugars of Asparagus officinalis Linn. Polysaccharide by CZE with Amperometric Detection. Chromatographia 75, 297–304 (2012). https://doi.org/10.1007/s10337-012-2197-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10337-012-2197-5

Keywords

Search

Navigation