Abstract
Bakanae disease is an important fungal disease in the world. No rice (Oryza sativa L.) varieties have been found to be completely resistant to this disease. To facilitate accurate, uniform and simultaneous screening of many rice accessions, we developed an inoculation method for microconidia of Fusarium fujikuroi using a tissue embedding cassette and seedling tray. Standards for evaluating the inoculated rice seedlings as healthy or unhealthy were also established. The method was fast and reproducible for accurately evaluating resistance to bakanae disease in rice.
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This study was supported by grants from the Rural Development Administration (PJ0086852013).
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M.-H. Kim, Y.-J. Hur and S. B. Lee contributed equally to this work.
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Kim, MH., Hur, YJ., Lee, S.B. et al. Large-scale screening of rice accessions to evaluate resistance to bakanae disease. J Gen Plant Pathol 80, 408–414 (2014). https://doi.org/10.1007/s10327-014-0528-0
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DOI: https://doi.org/10.1007/s10327-014-0528-0