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Characterization of Spleen Transcriptome of Schizothorax prenanti during Aeromonas hydrophila Infection

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Abstract

Schizothorax prenanti (S. prenanti) is an indigenous fish species and is popularly cultured in southwestern China. In recent years, intensive farming of S. prenanti and water quality deterioration has increased the susceptibility of this fish to various pathogens, including Aeromonas hydrophila (A. hydrophila), which has caused severe damage to S. prenanti production. However, the understanding of molecular immune response of S. prenanti to A. hydrophila infection is still lacking. In order to better comprehend the S. prenanti time series immune response process against A. hydrophila, we conducted the first transcriptomic comparison in S. prenanti spleen at 4, 24, and 48 h after the infection challenge of A. hydrophila against their control counterparts. In total, 628 million clean reads were obtained from 18 libraries and assembled into 262,745 transcripts. After eliminating sequence redundancy, 69,373 unigenes with an average length of 1476 bp were obtained. Comparative analysis revealed 1890 unigenes with significantly differential expression, including 172, 455, 589 upregulated and 27, 676, 551 unigenes downregulated genes for 4, 24, and 48 h post-infection, respectively. Differentially expressed genes (DEGs) were validated using qPCR for 15 randomly selected genes. Enrichment and pathway analysis of DEGs was carried out to understand the functions of the immune-related genes. Our results revealed that many important functional genes relating to complement and coagulation cascades, chemokine signaling pathway, toll-like receptor signaling pathway, NOD-like receptor signaling pathway and leukocyte transendothelial migration were regulated during the infection of A. hydrophila, and the expression of those genes reflected the transcriptome profiles during the challenging stages.

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Acknowledgments

We thank Lingbing Zeng, in Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences, and Bin Chen, in College of Animal Science and Technology, Hunan Agricultural University, for providing constructive suggestions on the experiments. We also thank Genhua Yue and Baoqing Ye, Molecular Population Genetics Group, Temasek Life Sciences Laboratory, 1 research link, National University of Singapore, for providing constructive suggestions on the manuscript.

Funding

This research was supported by the National Natural Science Foundation of China (31402302, 31602207), Fundamental Research Funds for the central Universities (XDJK2015C034, XDJK2017B008, XDJK2017C035), Scientific Research Initiation Project aided by a special fund, Southwest University Rongchang Campus (20700208), and the Youth Foundation of Southwest University Rongchang Campus (20700937, 20700938).

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  1. Hua Ye, Shijun Xiao and Hui Luo contributed equally to this work.

Authors and Affiliations

  1. College of Animal Science, Southwest University, Chongqing, 402460, China

    Hua Ye, Zhengshi Zhang, Chengke Zhu, Bingjie Hu, Shuming Zheng & Hui Luo

  2. Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Chongqing, 400175, China

    Hua Ye, Chengke Zhu, Shuming Zheng & Hui Luo

  3. College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128, China

    Xiaoqing Wang & Hui Luo

  4. Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde, 415000, China

    Xiaoqing Wang

  5. Key Laboratory of Healthy Mariculture for East China Sea of Agriculture Ministry, Fisheries College, Jimei University, Xiamen, 361021, China

    Shijun Xiao, Zhiyong Wang & Changhuan Lv

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  1. Hua Ye
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  2. Shijun Xiao
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Ye, H., Xiao, S., Wang, X. et al. Characterization of Spleen Transcriptome of Schizothorax prenanti during Aeromonas hydrophila Infection. Mar Biotechnol 20, 246–256 (2018). https://doi.org/10.1007/s10126-018-9801-0

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