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Decontamination of deep dentin by means of erbium, chromium:yttrium-scandium-gallium-garnet laser irradiation

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Abstract

The aim of this in vitro study was to evaluate the depth of effectiveness of erbium, chromium:yttrium-scandium-gallium-garnet (Er,Cr:YSGG) laser irradiation on microorganism reduction. From human roots, dentin slices of 100 μm to 1,000 μm thickness were prepared. These specimens were sterilized and then inoculated with 1 μl of Enterococcus faecalis suspension. The backs of the specimens were then irradiated with Er,Cr:YSGG radiation at a pulse energy of 3.13 mJ, delivered at an incidence angle of 5° to the dentin slice surface. A control group was left without irradiation. The remaining bacteria were collected in 1 ml sterilized NaCl solution, serially diluted and seeded in Columbia-Agar plates. Despite the low pulse energy of 3.13 mJ, the Er,Cr:YSGG laser irradiation resulted in significant bacterial reduction up to a dentin thickness of 500 μm (P < 0.05). Scanning electron microscopy (SEM) micrographs of the contaminated and irradiated surfaces showed the absence of a smear layer and opened dentinal tubules.

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Acknowledgments

The authors would to thank Biolase (San Clemente, CA, USA) for providing a Waterlase Millennium laser system and would like to express their gratitude to the German Academic Exchange Service (Deutsche Akademische Austauschdienst, DAAD) and CAPES. Grants process A/05/50718.

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Authors and Affiliations

  1. Department for Conservative Dentistry, Periodontology and Preventive Dentistry (ZPP), RWTH Aachen University, Pauwelsstr. 30, 52074, Aachen, Germany

    René Franzen, Jörg Meister, Anja Wallerang, Leon Vanweersch, Friedrich Lampert & Norbert Gutknecht

  2. Restorative Dentistry Department, Dentistry School of University of São Paulo (USP), São Paulo, SP, Brazil

    Marcella Esteves-Oliveira

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  1. René Franzen
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  2. Marcella Esteves-Oliveira
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  3. Jörg Meister
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  7. Norbert Gutknecht
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Correspondence to René Franzen.

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Franzen, R., Esteves-Oliveira, M., Meister, J. et al. Decontamination of deep dentin by means of erbium, chromium:yttrium-scandium-gallium-garnet laser irradiation. Lasers Med Sci 24, 75–80 (2009). https://doi.org/10.1007/s10103-007-0522-2

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  • DOI: https://doi.org/10.1007/s10103-007-0522-2

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