Skip to main content
SpringerLink
Log in

Extract of Makgeolli suldeot downregulates NF-κB-mediated MMP-9 expression, thereby inhibiting invasive activity in a human breast cancer cell line, MDA-MB-231

  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

Makgeolli is a Korean traditional alcoholic beverage prepared by fermenting rice starch using nuruk. It is known for its health benefits, but the mechanisms underlying its inhibitory effects on cancer remain unclear. Here a 70% ethanol extract of Makgeolli suldeot (70EMS) was tested for its inhibitory activities against cancer metastasis. This extract interrupted the formation of the neovasculature and reduced the migratory and invasive activity of breast cancer cells. Furthermore, it dose-dependently suppressed the activity of matrix metalloproteinase (MMP)-9 and phosphorylation of ERK1/2, JNK, and Akt. Moreover, 70EMS strongly inhibited the translocation of nuclear factor-kappa B (NF-κB) into the nucleus and expression of activator protein-1 (AP-1). These data indicate that the anti-invasive effect of 70EMS may be related to the inhibition of ERK1/2, phosphorylation of JNK, and reduced expression of NF-κB and AP-1, thereby attenuating MMP-9 in human breast cancer cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Duffy MJ, McGowan PM, Gallgher WM. Cancer invasion and metastasis: Changing view. J. Pathol. 214: 283–293 (2008)

    Article  CAS  Google Scholar 

  2. Bjorklund M, Koivunen E. Gelatinase-mediated migration and invasion of cancer cells. Biochim. Biophys. Acta 1755: 37–69 (2005)

    Google Scholar 

  3. Cortes-Reynosa P, Robledo T, Macias-Silva M, Wu SV, Salazar EP. Src kinase regulates metalloproteinase-9 secretion induced by type IV collagen in MCF-7 human breast cancer cells. Matrix Biol. 27: 220–231 (2008)

    Article  CAS  Google Scholar 

  4. Deryugina EI, Quigley JP. Matrix metalloproteinases and tumor metastasis. Cancer Metast. Rev. 25: 9–34 (2006)

    Article  CAS  Google Scholar 

  5. Sternlicht MD, Werb Z. How matrix metalloproteinases regulate cell behavior. Annu. Rev. Cell Dev. Bi. 17: 463–516 (2001)

    Article  CAS  Google Scholar 

  6. Egeblad M, Werb Z. New functions for the matrix metalloproteinases in cancer progression. Nat. Rev. Cancer 2: 161–174 (2002)

    Article  CAS  Google Scholar 

  7. Eberhardt W, Huwiler A, Beck KF, Walpen S, Pfeilschifter J. Amplification of IL-1 ß-induced matrix metalloproteinase-9 expression by superoxide in rat glomerular mesangial cells is mediated by increased activities of NF-ΚB and activating protein-1 and involves activation of the mitogen-activated protein kinase pathway. J. Immunol. 165: 5788–5797 (2000)

    Article  CAS  Google Scholar 

  8. Gum R, Wang H, Lengyel E, Juarez J, Boyd D. Regulation of 92 kDa type IV collagenase expression by the jun aminoterminal kinase-and the extracellular signal-regulated kinase-dependent signaling cascades. Oncogene 14: 1481–1493 (1997)

    Article  CAS  Google Scholar 

  9. Sato T, Koike L, Miyata Y, Hirata M, Mimaki Y, Sashida Y, Yano M, Ito A. Inhibition of activator protein-1 binding activity and phosphatidylinositol 3-kinase pathway by nobiletin, a polymethoxy flavonoid, results in augmentation of tissue inhibitor of metalloproteinase-1 production and suppression of production of matrix metalloproteinase-1 and -9 in human fibrosarcoma HT-1080 cells. Cancer Res. 62: 1025–1029 (2002)

    CAS  Google Scholar 

  10. Lee SO, Jeong YJ, Kim M, Kim CH, Lee IS. Suppression of PMA-induced tumor cell invasion by capillarisin via the inhibition of NF-ΚB-dependent MMP-9 expression. Biochem. Bioph. Res. Co. 366: 1019–1024 (2008)

    Article  CAS  Google Scholar 

  11. Duffy MJ, Maguire TM, Hill A, McDermott E, O’Higgins N. Metalloproteinases: Role in breast carcinogenesis, invasion and metastasis. Breast Cancer Res. 2: 252–257 (2000)

    Article  CAS  Google Scholar 

  12. Faqan-Solis KD, Schneider SS, Pentecost BT, Bentley BA, Otis CN, Gierthy JF, Arcaro KF. The RhoA pathway mediates MMP-2 and MMP-9-independent invasive behavior in a triple-negative breast cancer cell line. J. Cell. Biochem. 114: 1385–1394 (2013)

    Article  Google Scholar 

  13. Daniele A, Zito AF, Giannelli G, Divella R, Asselti M, Mazzocca A, Paradiso A, Quaranta M. Expression of metalloproteinases MMP-2 and MMP-9 in sentinel lymph node and serum of patients with metastatic and non-metastatic breast cancer. Anticancer Res. 30: 1–7 (2010)

    Google Scholar 

  14. Seo DH, Jung JH, Kim HY, Kim YR, Ha SJ, Kim YC, Park CS. Identification of lactic acid bacteria involved in traditional Korean rice wine fermentation. Food Sci. Biotechnol. 16: 994–998 (2007)

    CAS  Google Scholar 

  15. Lee SJ, Bae HJ, Ryu JY, Lee DY, Kim GW, Baek NI, Kwon MS, Hong SY. Extracts from Rhizopus oryzae KSD-815 of Korean traditional Nuruk confer the potential to inhibit hypertension, platelet aggregation, and cancer metastasis in vitro. Food Sci. Biotechnol. 18: 1423–1429 (2009)

    Google Scholar 

  16. Lee GH. The properties and new technologies of Korean medicinal wine and Takju. J. Microbiol. Biotechn. 7: 4036–4046 (1994)

    Google Scholar 

  17. Lee JS, Lee TS, Park SO, Noh BS. Quality characteristics in mash of Takju prepared by different raw materials. Korean J. Food Sci. Technol. 28: 330–336 (1996)

    Google Scholar 

  18. Hong YH, Bae SH, Jung EY, Son HS, Shin KS, Kwon KH, Suh HJ. Radical scavenging activities of Korean traditional rice wine, Takju. J. Food Sci. Nutr. 14: 109–115 (2009)

    Article  CAS  Google Scholar 

  19. Kim MH, Kim WH, Bae SJ. The effect of Makkoli on serum lipid concentration in male rats. J. Nat. Sci. Silla Univ. 9: 73–84 (2001)

    CAS  Google Scholar 

  20. Shin MO, Kang DY, Kim MH, Bae SJ. Effect of growth inhibition and quinine reductase activity stimulation of Makgeoly fractions in various cancer cells. Kor. Soc. Food Sci. Nutr. 37: 288–293 (2008)

    Article  Google Scholar 

  21. Albini A, Iwamoto Y, Kleinman HK, Martin GR, Aaronson SA, Kozlowski JM, McEwan RN. A rapid in vitro assay for quantitating the invasive potential of tumor cells. Cancer Res. 17: 3239–3245 (1987)

    Google Scholar 

  22. Hanahan D, Folkman J. Patterns and emerging mechanisms of the angiogenic switch during tumorigenesis. Cell 86: 353–364 (1996)

    Article  CAS  Google Scholar 

  23. Chiu CT, Chen JH, Chou FP, Lin HH. Hibiscus sabdariffa Leaf inhibits human prostate cancer cell invasion via down-regulation of Akt/NF-ΚB/MMP-9 pathway. Nutrients 7: 5065–5087 (2015)

    Article  Google Scholar 

  24. Dhillon AS, Hagan S, Rath O, Kolch W. MAP kinase signaling pathways in cancer. Oncogene 26: 3279–3290 (2007)

    Article  CAS  Google Scholar 

  25. Karin M, Greten FR. NF-ΚB: Linking inflammation and immunity to cancer development and progression. Nat. Rev. Immunol. 5: 749–759 (2005)

    Article  CAS  Google Scholar 

  26. Whitmarsh AJ, Davis RJ. Transcription factor AP-1 regulation by mitogen-activated protein kinase signal transduction pathway. J. Mol. Med. 74: 589–604 (1996)

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women’s University, Seoul, 04310, Korea

    Sang-Jin Lee & Gyu-Un Bae

  2. Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin, Gyeonggi, 17104, Korea

    Sunyoung Park & Cheon-Seok Park

  3. Research Laboratories, Kooksoondang Brewery Co., Ltd., Seongnam, Gyeonggi, 13202, Korea

    Sunyoung Park

  4. Biological and Genetic Resources Assessment Division, National Institute of Biological Resources, Incheon, 22689, Korea

    Hye Yoon Park & Changmu Kim

  5. Brewing Research Center, Academic Industry Cooperation, Hankyong National University, Anseong, Gyeonggi, 17579, Korea

    Gye-Won Kim

Authors
  1. Sang-Jin Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sunyoung Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gyu-Un Bae
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hye Yoon Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Changmu Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Cheon-Seok Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Gye-Won Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gye-Won Kim.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lee, SJ., Park, S., Bae, GU. et al. Extract of Makgeolli suldeot downregulates NF-κB-mediated MMP-9 expression, thereby inhibiting invasive activity in a human breast cancer cell line, MDA-MB-231. Food Sci Biotechnol 25, 893–898 (2016). https://doi.org/10.1007/s10068-016-0146-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-016-0146-z

Keywords

Search

Navigation