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Rab32 and the remodeling of the imaginal midgut in Helicoverpa armigera

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Abstract

Midgut remodeling is a complex physiological process in holometabolous insects. During midgut remodeling, the larval midgut is decomposed by apoptosis or autophagy during metamorphosis, and the degraded larval midgut is partially absorbed as nutrients by the imaginal midgut for its formation. The molecular mechanism involved in this process is not clear. Here, we found that a Rab protein, which we have named HaRab32, is related to the organogenesis of insect imaginal midgut. Results show that HaRab32 is up-regulated in epidermis and midgut during metamorphosis. Its expression could be up-regulated by 20E. Immunohistochemistry shows Rab32 is distributed in the epithelium of the imaginal midgut during metamorphosis. Knockdown of HaRab32 by RNA interference disturbs the formation of the imaginal midgut. These data imply HaRab32 plays important roles in midgut remodeling by participating in the imaginal midgut formation.

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Acknowledgments

This work was supported by grants from the National Basic Research Program of China (2006CB102001) and the National Natural Science Foundation of China (No. 30710103901). We thank Dr. Marek Jindra and Masako Asahina in Biology Center, Czech Academy of Sciences and Department of Molecular Biology, University of South Bohemia, Czech Republic, for providing the plasmid L4440 and the HT115(DE3).

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Authors and Affiliations

  1. School of Life Sciences, Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, Shandong University, Jinan, 250100, Shandong, China

    Li Hou, Jin-Xing Wang & Xiao-Fan Zhao

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  1. Li Hou
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  2. Jin-Xing Wang
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  3. Xiao-Fan Zhao
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Correspondence to Xiao-Fan Zhao.

Additional information

The nucleotide sequence reported in this paper has been submitted to GenBank with accession number: HM037181.

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726_2010_720_MOESM1_ESM.tif

Analysis of the expressed and purified the recombinant HaRab32 protein. A. 12.5% SDS-PAGE, Lane 1; low-molecular-weight marker, Lane 2; protein extracts from E. coli before IPTG induction, Lane 3; extracts of E. coli after induced with IPTG for 3 h, Lane 4; purified HaRab32. B. Western blot, Lane 1, showing the specificity of the antiserum against HaRab32 from the midgut of the larvae at the metamorphic stage (100 µg midgut protein was used)

726_2010_720_MOESM2_ESM.tif

Nucleotide and deduced amino acid sequences of HaRab32. The arrows indicate the forward and reverse primers for RT-PCR. The sequence in gray indicates the Rab subfamily domain. The panes show the PKC phosphorylation site. The stop codon is indicated by an asterisk

726_2010_720_MOESM3_ESM.tif

Phylogenetic analysis of Rab32. A. Alignment of Rab32 proteins from various species. B. Phylogenetic analysis of HaRab32 with orthologs known from other species: A. aegypti XP_001652315, B. mori NP_001040211, C. quinquefasciatus XP_001862890, D. melanogaster BAA88238, D. rerio AAH66502, H. sapiens NP_006825, Mus musculus AAH16409, Synthetic construct AAV38818, X. laevis NP_001090131, C. familiaris XP_850212, H. sapiens NP_071732, R. norvegicus NP_665717, Bos Taurus NP_001092564

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Hou, L., Wang, JX. & Zhao, XF. Rab32 and the remodeling of the imaginal midgut in Helicoverpa armigera. Amino Acids 40, 953–961 (2011). https://doi.org/10.1007/s00726-010-0720-2

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