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The promoting role of an isolate of dark-septate fungus on its host plant Saussurea involucrata Kar. et Kir.

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Abstract

A dark-septate endophytic (DSE) fungus EF-37 was isolated from the roots of Saussurea involucrata Kar. et Kir., an endangered Chinese medicinal plant. The molecular identification of the fungus was based on internal transcribed spacer regions and the result showed that EF-37 was congeneric to Mycocentrospora. This study was conducted to clarify the influence of the root endophyte EF-37 on the host plant S. involucrata using material grown in a sterile culture bottle. After cultivation for 40 days, fungal hyphae were found to be branching repeatedly and forming “hyphae nets” in the epidermal layers. Significant differences were detected between the study groups in plant dry weight, plant height, root dry weight, shoot dry weight, and the number of hair root tips. There was a positive effect of endophyte EF-37 on plant root development, with results showing that cortical cells dissolved and formed aerate structures. There was a positive effect of endophyte EF-37 on plant growth, but chlorophyll fluorescence analysis showed that there were no significant differences between the study groups. In addition, analysis of the chemical composition of seedlings showed that the level of rutin was higher in plants cultivated with the EF-37 fungus compared to the controls. This study helps to establish a basis for germplasm conservation and for further investigation of the interaction between dark-septate fungi and this alpine plant.

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References

  • Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein data base search programs. Nucleic Acids Res 25:3389–3402. doi:10.1093/nar/25.17.3389

    Article  CAS  PubMed  Google Scholar 

  • Avdiushko SA (1993) Detection of several enzymatic activities in leaf prints cucumber plant. Physiol Mol Plant Pathol 42:441–454. doi:10.1006/pmpp.1993.1033

    Article  CAS  Google Scholar 

  • Bacon CW (1993) Abiotic stress tolerances (moisture, nutrients) and photosynthesis in endophyte-infected tall fescue. Agric Ecosyst Environ 44:123–142. doi:10.1016/0167-8809(93)90042-N

    Article  Google Scholar 

  • Beaudoin-Eagan LD (1985) Tyrosine and phenylalanine ammonia lyase activities during shoot initiation in tobacco callus cultures. Plant Physiol 78:438–441. doi:10.1104/pp.78.3.438

    Article  CAS  PubMed  Google Scholar 

  • Brundrett MC (2002) Coevolution of roots and mycorrhizas of land plants. New Phytol 154:275–304. doi:10.1046/j.1469-8137.2002.00397.x

    Article  Google Scholar 

  • Cousin MA (1996) Chitin as a measure of mold contamination of agricultural commodities and foods. J Food Prot 59:73–81

    CAS  Google Scholar 

  • Dau H (1994) Molecular mechanisms and quantitative models of variable photosystem II fluorescence. Photochem Photobiol 60:1–23

    Article  CAS  Google Scholar 

  • Demming B, Winter K (1987) Photoinhibition and zeaxanthin formation in intact leaves. Plant Physiol 84:218–224. doi:10.1104/pp.84.2.218

    Article  Google Scholar 

  • Feder N, O’Brien TP (1968) Plant microtechnique: some principles and new methods. Am J Bot 55:123–142. doi:10.2307/2440500

    Article  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791. doi:10.2307/2408678

    Article  Google Scholar 

  • Fernando AA, Currah RS (1996) A comparative study of the effects of the root endophytes Leptodontidium orchidicola and Phialocephala fortinii (fungi imperfecti) on the growth of some subalpine plants in culture. Can J Bot 74:1071–1078. doi:10.1139/b96-131

    Article  Google Scholar 

  • Fu LG (1992) China plant red data book—rare and endangered plants, vol 1. Chinese Science Press, Beijing, pp 234–235

    Google Scholar 

  • Fu CX, Xu YJ, Zhao DX, Ma FS (2005) A comparison between hairy root cultures and wild plants of Saussurea involucrata in phenylpropanoids production. Plant Cell Rep 24:750–754. doi:10.1007/s00299-005-0049-6

    Article  PubMed  CAS  Google Scholar 

  • Fumiaki U, Kazuhiko N (2007) A mutualistic symbiosis between a dark septate endophytic fungus, Heteroconium chaetospira, and a nonmycorrhizal plant, Chinese cabbage. Mycologia 99:175–184. doi:10.3852/mycologia.99.2.175

    Article  Google Scholar 

  • Guo SX, Xu JT (1990a) Studies on the cell ultrastructure in the course of gastrodia elata digesting Mycena osmundicola Lange and Armillaria mellea FR. Mycosystema 3:218–225

    Google Scholar 

  • Guo SX, Xu JT (1990b) Studies on the changes of cell ultrastructure in the course of seed germination of Bletilla striata under fungus infection conditions. Zhíwùxué Bào 8:594–598

    Google Scholar 

  • Guo B, Gao M, Liu CZ (2007) In vitro propagation of an endangered medicinal plant Saussurea involucrata Kar. et Kir. Plant Cell Rep 26:261–265. doi:10.1007/s00299-006-0230-6

    Article  CAS  PubMed  Google Scholar 

  • Henson JM, Butler MJ, Day AW (1999) The dark side of the mycelium: melanins of pathogenic fungi. Annu Rev Phytopathol 37:447–471. doi:10.1146/annurev.phyto.37.1.447

    Article  CAS  PubMed  Google Scholar 

  • Huang ZA, Jiang DA, Yang Y, Sun JW, Jin SH (2004) Effects of nitrogen deficiency on gas exchange, chlorophyll fluorescence, and antioxidant enzymes in leaves of rice plants. Photosynthetica 42:357–364. doi:10.1023/B:PHOT.0000046153.08935.4c

    Article  CAS  Google Scholar 

  • Jumpponen A (2001) Dark septate endophytes—are they mycorrhizal? Mycorrhiza 11:207–211. doi:10.1007/s005720100112

    Article  Google Scholar 

  • Jumpponen A, Trappe JM (1998a) Dark septate endophytes: a review of facultative biotrophic root-colonizing fungi. New Phytol 140:295–310. doi:10.1046/j.1469-8137.1998.00265.x

    Article  Google Scholar 

  • Jumpponen A, Trappe JM (1998b) Dark-septate root endophytes: a review with special reference to facultative biotrophic symbiosis. New Phytol 140:295–310. doi:10.1046/j.1469-8137.1998.00265.x

    Article  Google Scholar 

  • Jumpponen A, Mattson KG, Trappe JM (1998) Mycorrhizal functioning of Phialocephala fortinii: interactions with soil nitrogen and organic matter. Mycorrhiza 7:261–265. doi:10.1007/s005720050190

    Article  CAS  Google Scholar 

  • Krause GH, Weis E (1991) Chlorophyll fluorescence and photosynthesis: The basics. Annu Rev Plant Physiol Plant Mol Biol 42:313–349

    Article  CAS  Google Scholar 

  • Larson KC, Whitham TG (1991) Manipulation of food resourses by a gall-forming aphid: the physiology of sink-source interactions. Oecologia 88:15–21. doi:10.1007/BF00328398

    Article  Google Scholar 

  • Li GH, Zhao RC (1989) Studies on pharmacological actions of Saussurea involucrata Kar. et Kir. Acta Pharmacol Sin 15:368–369

    Google Scholar 

  • Li JS, Cai SQ (1998) Chemical constituents and pharmacological activity of eight species herb Xuelianhua. Chin Pharmaceut J 33:449–457

    CAS  Google Scholar 

  • Li Y, Wang CL, Guo SX, Yang JS, Xiao PG (2007) Three guaianolides from Saussurea involucrata and their contents determination by HPLC. J Pharm Biomed Anal 44:288–292. doi:10.1016/j.jpba.2007.02.017

    Article  CAS  PubMed  Google Scholar 

  • Liu LS, Xiao XH, Zhang LD (1985) Effect of the flavonoids from Saussurea involucrata on DNA synthesis of cancer cells. J Lanzhou Univ Nat Sci 21:80–83

    CAS  Google Scholar 

  • LoBuglio KF, Berbee ML, Taylor JW (1996) Phylogenetic origins of the asexual mycorrhizal symbiont Cenococcum geophilum FR. and other mycorrhizal fungi among the ascomycetes. Mol Phylogenet Evol 6:287–294. doi:10.1006/mpev.1996.0077

    Article  CAS  PubMed  Google Scholar 

  • Mada RJ, Bagyaraj DJ (1993) Root exudation from Leucaena leucocephala in relation to mycorrhizal colonization. World J Microbiol Biotechnol 9:342–344. doi:10.1007/BF00383076

    Article  Google Scholar 

  • Marks S, Clay K (1996) Physiological responses of Festuca arundinacea to fungal endophyte infection. New Phytol 133:727–733. doi:10.1111/j.1469-8137.1996.tb01941.x

    Article  Google Scholar 

  • Maxwell K, Johnson GN (2000) Chlorophyll fluorescence-a practical guide. J Exp Bot 51:659–668

    Article  CAS  PubMed  Google Scholar 

  • Money NP, The-Can Caesar-TonThat P, Frederick B, Henson JM (1998) Melanin synthesis is associated with changes in hyphopodial turgor, permeability, and wall rigidity in Gaeumannomyces graminis var. graminis. Fungal Genet Biol 24:240–251. doi:10.1006/fgbi.1998.1052

    Article  CAS  PubMed  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497. doi:10.1111/j.1399-3054.1962.tb08052.x

    Article  CAS  Google Scholar 

  • Page RDM (1996) An application to display phylogenetic tree personal computers. Comput Appl Biosci 12:357–358

    CAS  PubMed  Google Scholar 

  • Petrini O (1991) Fungal endophytes of tree leaves. In: Andrews JH, Hirano SS (eds) Microbial ecology of leaves. Springer, New York, pp 179–197

    Google Scholar 

  • Read DJ, Haselwandter K (1981) Observation on the mycorrhizal status of some alpine plant communities. New Phytol 88:341–352. doi:10.1111/j.1469-8137.1981.tb01729.x

    Article  Google Scholar 

  • Schulz B, Ak R, Dammann U, Aust HJ, Strack D (1999) Fungal symbiosis: from mutualism to parasitism, who controls the outcome, host or invader? New Phytol 151:705–716

    Google Scholar 

  • Shivanna MB, Meera MS, Hyakumachi M (1994) Sterile fungi from zoysiagrass rhizospere as plant growth promoters in spring wheat. Can J Microbiol 40:637–644

    Article  Google Scholar 

  • Swofford DL (1998) PAUP*4.0. Phylogenetic analysis using parsimony. Sinauer Associates, Sunderland

    Google Scholar 

  • Tan RX, Lu H, Wolfender JL, Yu TT, Zheng WF, Yang L, Gafner S, Hostettmann K (1999) Mono- and sesquiterpenes and antifungal constituents from Artemisia species. Planta Med 65:64–67. doi:10.1055/s-1999-13965

    Article  CAS  PubMed  Google Scholar 

  • Tan XM, Guo SX, Zhou YQ, Miu JH (2006) Microstructure and endophytic fungus distribution of Maytenus confertiflorus root. Chin Bull Bot 4:368–373

    Google Scholar 

  • White TJ, Bruns TD, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Academic, New York, pp 315–322

  • Wilcox HE, Wang CJK (1987) Mycorrhizal and pathological associations of dematiaceous fungi in roots of 7-month-old tree seedlings. Can J For Res 17:884–889. doi:10.1139/x87-140

    Article  Google Scholar 

  • Woerdenbag HJ, Merfort I, Passreiter CM, Schmidt TJ, Willuhn G, Van Uden W, Pras N, Kampinga HH, Konings AWT (1994) Cytotoxicity of flavonoids and sesquiterpene lactones from Arnica species against the GLC4 and the COLO 320 cell lines. Planta Med 60:434–437. doi:10.1055/s-2006-959526

    Article  CAS  PubMed  Google Scholar 

  • Wu LQ, Guo SX (2008) Interaction between an isolate of dark-septate fungi and its host plant Saussurea involucrata. Mycorrhiza 18:79–85. doi:10.1007/s00572-007-0159-9

    Article  CAS  PubMed  Google Scholar 

  • Zhang JH, Wang CL, Guo SX, Chen JM, Xiao PG (1999) Studies on the plant hormones produced by 5 species of endophytic fungi isolated from medicinal plants (Orchidacea). Acta Academiae Medicinae Sinicae 21:460–465

    CAS  PubMed  Google Scholar 

  • Zhao DX, Qiao CL, Wang Y (1998) Cell culture and selection of high flavonoids-producing cell lines in Saussurea medusa. Acta Bot Sin 40:515–520

    CAS  Google Scholar 

  • Zhao DX, Huang Y, Jin Z, Qu W, Lu D (2003) Effect of aggregate size in cell cultures of Saussurea medusa on cell growth and jaceosidin production. Plant Cell Rep 21:1129–1133. doi:10.1007/s00299-003-0631-8

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

The authors acknowledge the financial support from “the project funded by Basic Research Operation Cost of Central Public Welfare Research Institutes,” project number YZ-1-15.

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Authors and Affiliations

  1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 151, Malianwa North Road, Haidian District, Beijing, 100193, People’s Republic of China

    Li-qin Wu, Ya-li Lv, Zhi-xia Meng, Juan Chen & Shun-Xing Guo

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  1. Li-qin Wu
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  2. Ya-li Lv
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Correspondence to Shun-Xing Guo.

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Li-qin Wu and Ya-li Lv contributed equally to this work.

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Wu, Lq., Lv, Yl., Meng, Zx. et al. The promoting role of an isolate of dark-septate fungus on its host plant Saussurea involucrata Kar. et Kir.. Mycorrhiza 20, 127–135 (2010). https://doi.org/10.1007/s00572-009-0268-8

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