Abstract
Defensins are antimicrobial peptides that are produced by leukocytes and epithelial cells. Recent advances indicate that these peptides play an important role in innate immune responses. Nonetheless, the role of defensins in caprine eimeriosis remains unknown. Therefore, this study investigated the expression of a goat β-defensin, named GBD-2 in caprine intestinal epithelial cells (CIEC) stimulated with recombinant bovine interferon-gamma (IFN-γ) in the presence or absence of recombinant bovine interleukin-4 (IL-4) by a reverse transcriptase-polymerase chain reaction (RT-PCR) assay. GBD-2 mRNA was clearly expressed in IFN-γ-stimulated CIEC. On the other hand, the direct addition of IL-4 showed no significant effect on GBD-2 expression in CIEC. However, when supernatants from peripheral blood mononuclear cells (PBMC) cultured with IL-4 were added to CIEC, the expression of GBD-2 decreased. To elucidate if IFN-γ functions as a signaling molecule that facilitates the generation of GBD-2 against Eimeria spp. in goats, anti- IL-4 was added to PBMC from Eimeria-infected goats and levels of IFN-γ in culture supernatants were determined by an enzyme-linked immunosorbent assay test. Results showed that IFN-γ secretion increased when anti-IL-4 was added to PBMC. It then appears safe to suggest that IL-4 may be a further factor in the pathogenesis of goat coccidiosis and its induction may be part of an evasion strategy of the parasite to avoid pro-inflammatory responses.
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References
Agyei Ad, Odonkor M, Osei-Somuah A (2004) Concurrence of Eimeria and helminth parasitic infections in West African Dwarf kids in Ghana. Small Rumin Res 51:29–35
Bajaj-Elliott M, Fedeli P, Smith GV, Domizio P, Maher L, Ali RS, Quinn AG, Farthing MJ (2002) Modulation of host antimicrobial peptide (beta-defensins 1 and 2) expression during gastritis. Gut 51:356–361
Boniotto M, Jordan WJ, Eskdale J, Tossi A, Antcheva N, Crovella S, Connell ND, Gallagher G (2006) Human β-defensin 2 induces a vigorous cytokine response in peripheral blood mononuclear cells. Antimicrob Agents Chemother 50:1433–1441
Cunliffe RN, Mahida YR (2004) Expression and regulation of antimicrobial peptides in the gastrointestinal tract. J Leukoc Biol 75:49–58
Eckmann L (2003) Mucosal defences against Giardia. Parasite Immunol 25:259–270
Hart PH, Cooper RL, Finley-Jones JJ (1991) IL-4 suppresses IL-lβ, TNF-α and PGE2 production by human peritoneal macrophages. Immunology 72:344–349
Harwig SS, Swiderek KM, Kokryakov VN, Tan L, Lee TD, Panyutich EA, Aleshin GM, Shamova OV, Lehrer RI (1994) Gallinacins: cysteine-rich antimicrobial peptides of chicken leukocytes. FEBS Lett 342:281–285
Hashim A, Clyne M, Mulcahy G, Akiyoshi D, Chalmers R, Bourke B (2004) Host cell tropism underlies species restriction of human and bovine Cryptosporidium parvum genotypes. Infect Immun 72:6125–6131
Hériveau C, Dimier-Poisson I, Lowenthal J, Naciri M, Quéré P (2000) Inhibition of Eimeria tenella replication after recombinant IFN-γ activation in chicken macrophages, fibroblasts and epithelial cells. Vet Parasitol 92:37–49
Hermosilla C, Barbisch B, Heise A, Kowalik S, Zahner H (2002) Development of Eimeria bovis in vitro: suitability of several bovine, human and porcine endothelial cell lines, bovine fetal gastrointestinal, Madin–Darby bovine kidney (MDBK) and African green monkey kidney (VERO) cells. Parasitol Res 88:301–307
Huttner KM, Bevins CL (1999) Antimicrobial peptides as mediators of epithelial host defense. Pediatr Res 45:785–794
Huttner KM, Kozak CA, Bevins CL (1997) The mouse genome encodes a single homolog of the antimicrobial peptide human beta-defensin 1. FEBS Lett 413:45–49
Koudela B, Boková A (1998) Coccidiosis in goats in the Czech Republic. Vet Parasitol 76:261–267
Kulkarni MM, McMaster WR, Kamysz E, Kamysz W, Engman DM, McGwire BS (2006) The major surface-metalloprotease of the parasitic protozoan, Leishmania, protects against antimicrobial peptide-induced apoptotic killing. Mol Microbiol 62:1484–1497
Lillehoj HS, Min W, Dalloul RA (2004) Recent progress on the cytokine regulation of intestinal immune responses to Eimeria. Poult Sci 83:611–623
Luenser K, Fickel J, Ludwig A (2005) Evolution of caprine and ovine β-defensin genes. Immunogenetics 57:487–498
Nomura I, Goleva E, Howell MD, Hamid QA, Ong PY, Hall CF, Darst MA, Gao B, Boguniewicz M, Travers JB, Leung DY (2003) Cytokine milieu of atopic dermatitis, as compared to psoriasis, skin prevents induction of innate immune response genes. J Immunol 171:3262–3269
Norton CC (1986) Coccidia of domestic goats, Capra hircus, with notes on Eimeria ovinoidalis and E. bakuenis (E. ovina) from sheep Ovis aries. Parasitology 92:279–289
O’Neil DA, Martin Porter E, Elewaut DG, Anderson M, Eckmann L, Ganz T, Kagnoff MF (1999) Expression and regulation of the human beta-defensins hBD-1 and hBD-2 in intestinal epithelium. J Immunol 163:6718–6728
Ovington KS, Alleva LM, Kerr EA (1995) Cytokines and immunological control of Eimeria spp. Int J Parasitol 25:1331–1351
Pedersen LG, Castelruiz Y, Jacobsen S, Aasted B (2002) Identification of monoclonal antibodies that cross-react with cytokines from different animal species. Vet Immunol Immunopathol 88:111–122
Penzhorn BL, Rognlie MC, Hall LL, Knapp SE (1994) Enteric coccidian of Cashemire goats in southwestern Montana, USA. Vet Parasitol 55:137–142
Rose ME, Wakelin D, Hesketh P (1989) Gamma interferon controls Eimeria vermiformis primary infection in balb/c mice. Infect Immun 57:1599–1603
Schonwetter BS, Stolzenberg ED, Zasloff MA (1995) Epithelial antibiotics induced at sites of inflammation. Science 267:1645–1648
Steinfelder S, Lucius R, Greif F, Pogonka T (2005) Treatment of mice with the anticoccidial drug Toltrazuril does not interfere with the development of a specific cellular intestinal immune response to Eimeria falciformis. Parasitol Res 97:458–465
Tarver AP, Clark DP, Diamond G, Russell JP, Erdjument-Bromage H, Tempst P, Cohen KS, Jones DE, Sweeney RW, Wines M, Hwang S, Bevins CL (1998) Enteric beta-defensin: molecular cloning and characterization of a gene with inducible intestinal epithelial cell expression associated with Cryptosporidium parvum infection. Infect Immun 66:1045–1056 (Erratum in Infect Immun, 1998, 66:2399)
Varoga D, Paulsen FP, Kohrs S, Grohmann S, Lippross S, Mentlein R, Tillmann BN, Goldring MB, Besch L, Pufe T (2006) Expression and regulation of human beta-defensin-2 in osteoarthritic cartilage. J Pathol 209:166–173
Wakelin D, Rose ME, Hesketh P, Else KJ, Grencis RK (1993) Immunity to coccidiosis: genetic influences on lymphocyte and cytokine response to infection with Eimeria vermiformis in inbred mice. Parasite Immunol 15:11–19
Zaalouk TK, Bajaj-Elliott M, George JT, McDonald V (2004) Differential regulation of beta-defensin gene expression during Cryptosporidium parvum infection. Infect Immun 72:2772–2779
Zhao C, Nguyen T, Liu L, Shamova O, Brogden K, Lehrer RI (1999) Differential expression of caprine β-defensins in digestive and respiratory tissues. Infect Immun 67:6221–6224
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Ibarra-Velarde, F., Alcala-Canto, Y. Downregulation of the goat β-defensin-2 gene by IL-4 in caprine intestinal epithelial cells infected with Eimeria spp.. Parasitol Res 101, 613–618 (2007). https://doi.org/10.1007/s00436-007-0523-x
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DOI: https://doi.org/10.1007/s00436-007-0523-x