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Lateral diffusion of CO2 in leaves of the crassulacean acid metabolism plant Kalanchoë daigremontiana Hamet et Perrier

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Abstract

Dynamic patchiness of photosystem II (PSII) activity in leaves of the crassulacean acid metabolism (CAM) plant Kalanchoë daigremontiana Hamet et Perrier, which was independent of stomatal control and was observed during both the day/night cycle and circadian endogenous oscillations of CAM, was previously explained by lateral CO2 diffusion and CO2 signalling in the leaves [Rascher et al. (2001) Proc Natl Acad Sci USA 98:11801–11805; Rascher and Lüttge (2002) Plant Biol 4:671–681]. The aim here was to actually demonstrate the importance of lateral CO2 diffusion and its effects on localized PSII activity. Covering small sections of entire leaves with silicone grease was used for local exclusion of a contribution of atmospheric CO2 to internal CO2 via transport through stomata. A setup for combined measurement of gas exchange and chlorophyll fluorescence imaging was used for recording photosynthetic activity with a spatiotemporal resolution. When remobilization of malic acid from vacuolar storage and its decarboxylation in the CAM cycle caused increasing internal CO2 concentrations sustaining high PSII activity behind closed stomata, PSII activity was also increased in adjacent leaf sections where vacuolar malic acid accumulation was minimal as a result of preventing external CO2 supply due to leaf-surface greasing, and where therefore CO2 could only be supplied by diffusion from the neighbouring malic acid-remobilizing leaf tissue. This demonstrates lateral CO2 diffusion and its effect on local photosynthetic activity.

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Abbreviations

CAM :

Crassulacean acid metabolism

g H2O :

Leaf conductance for water vapour

J CO2 :

Net CO2 exchange

J H2O :

Net water vapour exchange

p aCO2 :

Atmospheric CO2 partial pressure in pascals

p iCO2 :

Internal CO2 concentration

Rubisco :

Ribulose-1,5-bisphosphate carboxylase/oxygenase

References

  • Beyschlag W, Eckstein J (1997) Stomatal patchiness. Prog Bot 59:283–298

    Google Scholar 

  • Cockburn W, Patel K (2002) Control of internal carbon dioxide concentration during decarboxylation in CAM plants. In: Holtum JAM (ed) CAM 2001. The IIIrd international congress on crassulacean acid metabolism, The School of Tropical Biology, James Cook University, Townsville, Queensland, p 12

  • Cowan IR (1977) Stomatal behaviour and environment. Adv Bot Res 117–228

  • Franks PJ, Farquhar GD (2001) The effects of exogenous abscisic acid on stomatal development, stomatal mechanics and leaf gas exchange in Tradescantia virginiana. Plant Physiol 125:935–942

    Article  CAS  PubMed  Google Scholar 

  • Frechilla S, Talbott LD, Zeiger E (2002) The CO2 response of Vicia guard cells acclimates to growth environment. J Exp Bot 53:545–550

    Article  CAS  PubMed  Google Scholar 

  • Grams TEE, Borland AM, Roberts A, Griffiths H, Beck F, Lüttge U (1997) On the mechanism of reinitiation of endogenous crassulacean acid metabolism by temperature changes. Plant Physiol 113:1309–1317

    CAS  PubMed  Google Scholar 

  • Hanstein SM (2002) CO2-triggered chloride release from guard cells in intact fava bean leaves. Kinetics of the onset of stomatal closure. Plant Physiol 130:940–950

    Article  CAS  PubMed  Google Scholar 

  • Hedrich R, Neimanis S, Savchenko G, Felle HH, Kaiser WM, Heber U (2001) Changes in apoplastic pH and membrane potential in leaves in relation to stomatal responses to CO2, malate, abscisic acid or interruption of water supply. Planta 213:594–601

    Article  CAS  PubMed  Google Scholar 

  • Huxman TE, Monson RK (2003) Stomatal responses of C3, C3–C4 and C4 Flaveria species to light and intercellular CO2 concentration: implications for the evolution of stomatal behaviour. Plant Cell Environ 26:313–322

    Article  CAS  Google Scholar 

  • Kluge M, Böhlke C, Queiroz O (1981) Crassulacean acid metabolism (CAM) in K. daigremontiana. Changes in intercellular CO2 concentration during a normal CAM cycle and during cycles in continuous light or darkness. Planta 152:87–92

    CAS  Google Scholar 

  • Lüttge U (2002) CO2-concentrating: consequences in crassulacean acid metabolism. J Exp Bot 53:2131–2142

    Article  PubMed  Google Scholar 

  • Lüttge U, Beck F (1992) Endogenous rhythms and chaos in crassulacean acid metabolism. Planta 188:28–38

    Google Scholar 

  • Lüttge U, Stimmel KH, Smith JAC, Griffiths H (1986) Comparative ecophysiology of CAM and C3 bromeliads. II. Field measurements of gas exchange of CAM bromeliads in the humid tropics. Plant Cell Environ 9:377–383

    Google Scholar 

  • Maxwell K, von Caemmerer S, Evans JR (1997) Is a low internal conductance to CO2 diffusion a consequence of succulence in plants with crassulacean acid metabolism. Aust J Plant Physiol 24:777–786

    CAS  Google Scholar 

  • Möllering H (1974) l-Malate. Bestimmung mit Malat-Dehydrogenase und Glutamat-Oxalacetat-Transaminase. In: Bermeyer HW (ed) Methoden der enzymatischen Analyse, Vol 25. Verlage Chemie, Weinheim pp 1636–1639

  • Portis AR (1992) Regulation of ribulose-1.5-bisphosphate carboxylase/oxygenase activity. Annu Rev Plant Physiol Plant Mol Biol 43:415–437

    Article  CAS  Google Scholar 

  • Rascher U, Lüttge U (2002) High-resolution chlorophyll fluorescence imaging serves as a non-invasive indicator to monitor the spatio-temporal variations of metabolism during the day–night cycle and during the endogenous rhythm in continuous light in the CAM plant Kalanchoë daigremontiana. Plant Biol 4:671–681

    Article  CAS  Google Scholar 

  • Rascher U, Hütt MT, Siebke K, Osmond B, Beck F, Lüttge U (2001) Spatiotemporal variation of metabolism in a plant circadian rhythm: the biological clock as an assembly of coupled individual oscillators. Proc Natl Acad Sci USA 98:11801–11805

    Article  CAS  PubMed  Google Scholar 

  • Schroeder JI, Allen GJ, Hugouvieux V, Kwak JM, Waner D (2001) Guard cell signal transduction. Annu Rev Plant Physiol Plant Mol Biol 52:627–658

    Article  CAS  PubMed  Google Scholar 

  • Willert DJ, Maryssek R, Herppick W (1995) Experimentielle Pflanzenökologie. Grundlagen und Anwendungen. Thieme, Stuttgart

  • Zeiger E, Talbott LD, Frechilla S, Srivastava A, Zhu J (2002) The guard cell chloroplast: a perspective for the twenty-first century. New Phytol 153:415–424

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Botany, Darmstadt University of Technology, Schnittspahnstrasse 3–5, 64287, Darmstadt, Germany

    Heitor M. Duarte & Ulrich Lüttge

  2. Institute of Applied Physics, Darmstadt University of Technology, Hochschulstrasse 4a, 64289, Darmstadt, Germany

    Ivona Jakovljevic & Friedemann Kaiser

Authors
  1. Heitor M. Duarte
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  2. Ivona Jakovljevic
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  3. Friedemann Kaiser
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  4. Ulrich Lüttge
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Correspondence to Ulrich Lüttge.

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Duarte, H.M., Jakovljevic, I., Kaiser, F. et al. Lateral diffusion of CO2 in leaves of the crassulacean acid metabolism plant Kalanchoë daigremontiana Hamet et Perrier. Planta 220, 809–816 (2005). https://doi.org/10.1007/s00425-004-1398-z

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  • DOI: https://doi.org/10.1007/s00425-004-1398-z

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