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Effect of low-power red light laser irradiation on the viability of human skin fibroblast

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Human skin fibroblast monolayers (S-126 cell line) were exposed to laser radiation (wavelength 670 nm, power density 40 mW/cm2). The energy densities were 2 J/cm2 and 12 J/cm2, respectively, and the irradiation was carried out at a temperature of 22°C. For fibroblast viability evaluation, the colorimetric assay (conversion of thiazolyl blue to formazan) was used. The experiments were carried out at 37°C, in the presence of 5% CO2, and at different time periods of incubation after irradiation (2, 4, 8 h and 1, 2, 3, 4, 5 days). The results indicated that there was a certain stimulating effect on the long-term proliferation of skin fibroblasts and that the stimulation proceeded in two stages, the first one 2 h and the second one 3 days post-irradiation.

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Received 7 January 1998 / Accepted in revised form: 11 June 1998

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Bednarska, K., Rózga, B., Kołodziejczyk, K. et al. Effect of low-power red light laser irradiation on the viability of human skin fibroblast. Radiat Environ Biophys 37, 215–217 (1998). https://doi.org/10.1007/s004110050120

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  • DOI: https://doi.org/10.1007/s004110050120

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