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Preparation, characterization of cationic terbium luminescent copolymer and its interaction with DNA

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Abstract

A complex of Tb3+, acrylic acid (AA), and 1, 10-phenanthroline (Phen) was synthesized. The structure and fluorescence of Tb(AA)3Phen·H2O was characterized with elemental analysis, FT-IR, and fluorescence spectroscopy. A novel copolymer containing rare earth complex, poly (METAC-co-NIPAm-co-Tb(AA)3Phen·H2O) (PMNTb), was prepared by free radical copolymerization in methanol with azodiisobutyronitrile (AIBN) as initiator. 1H NMR, fluorescence spectroscopy, UV-vis spectroscopy, and TEM were used to characterize this copolymer. The interaction of PMNTb with DNA was studied by fluorescence spectroscopy, UV-vis spectroscopy, and agarose gel electrophoresis. The results of fluorescence, UV-vis absorption, and agarose electrophoresis indicated that the PMNTb could interact with DNA in an electrostatic bonding mode and the bonding constant was 3.4 × 105 L mol−1. The TEM observation showed that the PMNTb could form micelles in water solution; the efficient complexation of PMNTb with DNA occurred. The results of in vitro cytotoxicity indicated that PMNTb had good biocompability. These results laid the foundation for the further study in potential gene detective reagent and gene delivery carrier.

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Acknowledgments

This work is supported by the National Natural Science Foundation of China (No. 50973027) and Specialized Research Fund for the Doctoral Program of Higher Education (No. 20094208110002).The authors are grateful to Prof. Gongwu Song and his group, Faculty of Chemistry and Chemical Engineering, for their help in fluorescence spectra determination; Prof. Lixin Ma and his group, Faculty of Biology Science, for their help in DNA complexation determination and in vitro cytotoxicity, respectively.

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Authors and Affiliations

  1. Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei University, Wuhan, People’s Republic of China, 430062

    Xiaoxi Hu, Penghui Li, Shuilin Wu & ZuShun Xu

  2. Division of Spine Surgery, Department of Orthopaedics and Traumatology, The University of Hong Kong, Pokfulam, Hong Kong, People’s Republic of China

    Xiaoxi Hu, Kelvin W. K. Yeung, Shuilin Wu & ZuShun Xu

  3. Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, People’s Republic of China

    Paul K. Chu, Shuilin Wu & ZuShun Xu

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  1. Xiaoxi Hu
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Correspondence to Xiaoxi Hu.

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Hu, X., Li, P., Yeung, K.W.K. et al. Preparation, characterization of cationic terbium luminescent copolymer and its interaction with DNA. Colloid Polym Sci 289, 1459–1468 (2011). https://doi.org/10.1007/s00396-011-2472-2

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  • DOI: https://doi.org/10.1007/s00396-011-2472-2

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