Abstract
Previous studies suggested that late-successional tannin-rich plant communities increase the amount of dissolved organic N (DON) relative to dissolved inorganic N (DIN) in decomposing litter. We devised an experiment to test this hypothesis by adding varying proportions of black spruce (Picea mariana) and tannin-rich Kalmia angustifolia litter to forest floor samples collected on six black spruce cutovers. An increasing proportion of Kalmia litter increased condensed tannin and total phenolic concentrations over the course of a 46-week incubation period. Mineral N concentrations did not vary among treatments in spite of much higher total N concentrations in Kalmia litter. This was more likely due to the formation of protein–tannin complexes rather than microbial immobilization of N, as indicated by the decline in available C with increasing Kalmia litter on two of the five sampling dates. There was a significant positive linear trend between the proportion of Kalmia litter and the DON/DIN ratio on one sampling date (week 13) only. Results suggest that the DON/DIN ratio is controlled by confounding factors (e.g., tannins bonding with non-extractable humus particles) and has limited value for describing ecological succession.
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References
Anderson JPE, Domsch KH (1978) A physiological method for the quantitative measurement of microbial biomass in soils. Soil Biol Biochem 10:215–221
Bradley RL, Fyles JW (1995) A kinetic parameter describing soil available carbon and its relationship to rate increase in C mineralization. Soil Biol Biochem 27:167–172
Bradley RL, Titus BD, Preston CP (2000) Changes to mineral N cycling and microbial communities in black spruce humus after additions of (NH4)2SO4 and condensed tannins extracted from Kalmia angustifolia and balsam fir. Soil Biol Biochem 32:1227–1240
Cabrera ML, Beare MH (1993) Alkaline persulfate oxidation for determining total nitrogen in microbial biomass extracts. Soil Sci Soc Am J 57:1007–1012
Gomez KA, Gomez AA (1984) Statistical procedures for agricultural research. Wiley, New York
Handley WRC (1961) Further evidence for the importance of residual leaf protein complexes in litter decomposition and the supply of nitrogen for plant growth. Plant Soil 15:37–73
Holub SM, Lajtha K (2004) The fate and retention of organic and inorganic N-15-nitrogen in an old-growth forest soil in western Oregon. Ecosystems 7:368–380
Inderjit Mallik AU (1996) The nature of interference potential of Kalmia angustifolia. Can J Forest Res 26:1899–1904
Joanisse GD, Bradley RL, Preston CM, Munson AD (2007) Soil enzyme inhibition by condensed litter tannins may drive ecosystem structure and processes: the case of Kalmia angustifolia. New Phytol 175:535–546
Kalra YP, Maynard DG (1991) Methods manual for forest soil and plant analysis. Forestry Canada, Northwest Region, Northern Forestry Centre, Edmonton
Kanerva S, Kitunen V, Kiikkila O, Loponen J, Smolander A (2006) Response of soil C and N transformations to tannin fractions originating from Scots pine and Norway spruce needles. Soil Biol Biochem 38:1364–1374
Kraus TEC, Dahlgren RA, Zasoski RJ (2003) Tannins in nutrient dynamics of forest ecosystems—a review. Plant Soil 256:41–66
Mallik AU (2003) Conifer regeneration problems in boreal and temperate forests with ericaceous understory: role of disturbance, seedbed limitation, and keystone species change. Crit Rev Plant Sci 22:341–366
Nierop KGJ, Verstraten J (2006) Fate of tannins in Corsican Pine litter. J Chem Ecol 32:2709–2719
Nierop KGJ, Preston CM, Verstraten JM (2006) Linking the B ring hydroxylation pattern of condensed tannins to C, N and P mineralization. A case study using four tannins. Soil Biol Biochem 38:2794–2802
Northup RR, Yu ZS, Dahlgren RA, Vogt KA (1995) Polyphenol control of nitrogen release from pine litter. Nature 377:227–229
Preston CM (1999) Condensed tannins of salal (Gaultheria shallon Pursh): a contributing factor to seedling “growth-check” on northern Vancouver Island? In: Gross GG, Hemingway RW, Yoshida T (eds) Plant polyphenols 2: Chemistry, Biology, Pharmacology, Ecology. Kluwer Academic/Plenum, New York, pp 825–841
Preston CM, Trofymow JA, Sayer BG, Niu JN (1997) C-13 nuclear magnetic resonance spectroscopy with cross-polarization and magic-angle spinning investigation of the proximate-analysis fractions used to assess litter quality in decomposition studies. Can J Bot 75:1601–1613
Soil Classification Working Group (1998) The Canadian System of Soil Classification, 3rd ed. Agriculture and Agri-Food Canada, Research Branch, Publication 1646
Verkaik E, Jongkind AG, Berendse F (2006) Short-term and long-term effects of tannins on nitrogen mineralisation and litter decomposition in kauri (Agathis australis (D. Don) Lindl.) forests. Plant Soil 287:337–345
Waterman PG, Mole S (1994) Analysis of phenolic plant metabolites. Blackwell Scientific, Boston
Acknowledgments
We are grateful to P. Lebel and J. Lafond for technical assistance. Financial support was provided by a Strategic Project Grant and a Graduate Student Scholarship provided by the Natural Science and Engineering Research Council (NSERC) of Canada.
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Joanisse, G.D., Bradley, R.L. & Preston, C.M. Do late-successional tannin-rich plant communities occurring on highly acidic soils increase the DON/DIN ratio?. Biol Fertil Soils 44, 903–907 (2008). https://doi.org/10.1007/s00374-008-0297-z
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DOI: https://doi.org/10.1007/s00374-008-0297-z