Abstract.
API 50 CH fermentation profiles of 45 Lactobacillus, one Atopobium, and three Weissella strains incubated at 30°C and 37°C were evaluated. Atopobium uli and ten species of Lactobacillus showed stable patterns despite the change in temperature. The rest of the type strains showed discrepancy between the two incubation temperatures: 18 strains lost, 12 additionally fermented another sugar, and 7 others fermented a different one in lieu. The variation was maximum in L. delbrueckii subsp. delbrueckii. L. malefermentans failed to ferment any of the substrates at 37°C. Majority of the food and plant-associated strains (mainly heterofermenters) retained distinctive traits at 30°C, while most of the animal-associated strains (mostly homofermenters) did so at 37°C. No general trend was observed; 30°C appeared to promote heterofermentation, while 37°C favored homofermenters. Use of API 50 CH profiles for taxonomic purpose in most lactobacilli appears reproducible if a specific temperature for a species is strictly followed.
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Received: 10 December 1999 / Accepted: 10 January 2000
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Nigatu, A., Ahrné, S. & Molin, G. Temperature-Dependent Variation in API 50 CH Fermentation Profiles of Lactobacillus Species. Curr Microbiol 41, 21–26 (2000). https://doi.org/10.1007/s002840010085
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DOI: https://doi.org/10.1007/s002840010085