Abstract
A novel polyhydroxyalkanoate (PHA)-producing bacterium, Jeongeupia sp. USM3 (JCM 19920) was isolated from the limestone soil at Gua Tempurung, Perak, Malaysia. This is the first report on the complete genome sequence for the genus Jeongeupia. This genome consists of a circular chromosome with a size of 3,788,814 bp and contains 3557 genes. Two PHA synthase (phaC) genes encoding for the key enzyme in the polymerization of PHA monomers and other PHA-associated genes were identified from the genome. Phylogenetic analysis of the PhaC protein sequences has revealed that both PhaC1 and PhaC2 of Jeongeupia sp. USM3 are categorized as Class I PHA synthases with 56% similarity to each other. Both of the PHA synthase genes of this isolate were cloned and heterologously expressed in a PHA mutant strain Cupriavidus necator PHB−4. The ability of the transformants to accumulate PHA showed that both PhaC1 and PhaC2 were functional.
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Acknowledgements
This work was supported by USM-RIKEN International Centre for Aging Science (URICAS) (1001/PBIOLOGI/870033). N.-A.A.Z. is grateful to the Public Service Department of Malaysia scholarship scheme. Authors thank Hui Pheng Kho for her contribution in experimental works and Dr. Todd Taylor (RIKEN, Japan) for his useful advice.
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Zain, NA.A., Ng, LM., Foong, C.P. et al. Complete Genome Sequence of a Novel Polyhydroxyalkanoate (PHA) Producer, Jeongeupia sp. USM3 (JCM 19920) and Characterization of Its PHA Synthases. Curr Microbiol 77, 500–508 (2020). https://doi.org/10.1007/s00284-019-01852-z
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DOI: https://doi.org/10.1007/s00284-019-01852-z