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Paenibacillus lutimineralis sp. nov., Isolated From Bentonite

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Abstract

Strain MBLB1234T was isolated from bentonite samples collected at Guryong mining area located in Pohang, Republic of Korea and was taxonomically characterized by a polyphasic approach. This strain was a Gram-stain-negative, motile, endospore-forming, facultative anaerobic, catalase-positive, oxidase-negative, and rod-shaped bacterium. Strain MBLB1234T was able to grow at 20‒45 °C (optimum, 37 °C), pH 6.0‒10.0 (optimum, 7.0–8.0), and 0‒5.0% (w/v) NaCl (optimum, 0.5%). Genome size was 6,497,679 bp with a G + C content of 46.4 mol %. The genome was predicted to contain 5233 protein-coding genes, and 135 rRNA genes consisted of 10 5S rRNAs, 10 16S rRNAs, 10 23S rRNAs, and 105 tRNAs. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain MBLB1234T clustered with Paenibacillus motobuensis JCM 12774T and P. aceti JCM 31170T with 98.3–98.5% and 97.2–97.4% sequencing similarity, respectively. The major fatty acids of strain MBLB1234T were anteiso-C15:0 (35.7%), anteiso-C17:0 (17.8%), iso-C17:0 (14.5%), and C16:0 (11.0%). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, and one unidentified phospholipid, six unidentified aminophospholipids, and one unidentified lipid. The predominant isoprenoid quinone was menaquinone-7. DNA–DNA hybridization values between strain MBLB1234T and P. motobuensis JCM 12774T and P. aceti JCM 31170T were 34 and 38%, respectively. Average nucleotide identity value between strains MBLB1234T and P. aceti L14T was 82.3%. Based on characteristics of genomic, phenotypic, chemotaxonomic, and phylogenetic analyses, strain MBLB1234T represents a novel species of the genus P. , for which the name P. lutimineralis sp. nov. is proposed. The type strain is MBLB1234T (= JCM 32684T = KCTC 33978T).

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Acknowledgements

This research was supported by the Basic Research Project (18-3214) of the Korea Institute of Geoscience and Mineral Resources (KIGAM) funded by the Ministry of Science and ICT. This research was also supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2016R1D1A1B03931582), and the World Institute of Kimchi (KE1902-2) funded by the Ministry of Science, ICT & Future Planning.

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Authors and Affiliations

  1. Department of Bioengineering and Nano-Bioengineering, Graduate School of Incheon National University, Incheon, 22012, Republic of Korea

    Eui-Sang Cho & Myung-Ji Seo

  2. Microbiology and Functionality Research Group, World Institute of Kimchi, Gwangju, 61755, Republic of Korea

    In-Tae Cha & Seong Woon Roh

  3. Research Group of Healthcare, Korea Food Research Institute, Wanju, 55365, Republic of Korea

    Dong-Ho Seo & Young-Do Nam

  4. Advanced Geo-materials R&D Department, Pohang Branch, Korea Institute of Geoscience and Mineral Resources, Pohang, 37559, Republic of Korea

    Jae-Hwan Kim

  5. Division of Bioengineering, Incheon National University, Incheon, 22012, Republic of Korea

    Myung-Ji Seo

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The GenBank/EMBL/DDBJ accession number for genome and the Digital Protologue Database Taxon Number for of strain MBLB1234T are CP034346 and TA00598, respectively.

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Cho, ES., Cha, IT., Seo, DH. et al. Paenibacillus lutimineralis sp. nov., Isolated From Bentonite. Curr Microbiol 76, 995–1002 (2019). https://doi.org/10.1007/s00284-019-01710-y

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