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Mucilaginibacter ginsenosidivorans sp. nov., Isolated from Soil of Ginseng Field

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Abstract

A Gram-reaction-negative, aerobic, nonmotile, nonspore-forming, and rod-shaped bacterial strain designated Gsoil 3017T was isolated from soil of ginseng field and investigated by phenotypic and phylogenetic analyses. Strain Gsoil 3017T grew at 10–37 °C (optimal growth at 30 °C) and at pH 5.5–8.0 (optimal growth at pH 7) on R2A and nutrient agar without additional NaCl as a supplement. Strain Gsoil 3017T possessed β-glucosidase activity, which was responsible for its ability to transform ginsenosides Rb1, Rc, and Rd (the three dominant active components of ginseng) to F2 and C-K, respectively. Based on 16S rRNA gene phylogeny, the novel strain represents a new branch within the genus Mucilaginibacter family Sphingobacteriaceae, and clusters with Mucilaginibacter frigoritolerans FT22T (95.6%) and Mucilaginibacter gotjawali SA3-7T (95.6%). The G+C content of the genomic DNA was 48.7%. The predominant respiratory quinone was MK-7, and the major fatty acids were iso-C15:0, iso-C17:0 3-OH, and summed feature 3 (comprising C16:1 ω6c and/or C16:1 ω7c). The major polar lipid was phosphatidylethanolamine. Strain Gsoil 3017T could be differentiated genotypically and phenotypically from other type strains of the genus Mucilaginibacter. The isolate therefore represents a novel species, for which the name Mucilaginibacter ginsenosidivorans sp. nov. is proposed, with the type strain Gsoil 3017T (=KACC 14954T = JCM 17081T).

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Acknowledgements

This work was carried out with the support of "Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ012283)" Rural Development Administration and by the project on survey and excavation of Korean indigenous species of the National Institute of Biological Resources (NIBR) under the Ministry of Environment and by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2015R1C1A1A01054292), Republic of Korea. There is no person employed by the funders, but they extended support to the authors and this research work.

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Authors and Affiliations

  1. Department of New Biology, Daegu Gyeongbuk Institute of Science & Technology (DGIST), 333 Techno Jungang-daero, Hyeonpung-myeon, Dalseong gun, Daegu, 42988, Republic of Korea

    Minseok M. Kim

  2. Department of Biotechnology, Hankyong National University, 327 Chungang-no Anseong-si, Kyonggi-do, 17579, Republic of Korea

    Muhammad Zubair Siddiqi & Wan-Taek Im

  3. Center for Genetic Information Graduate School of Bio and Information Technology, Hankyong National University, 327 Chungang-no Anseong-si, Kyonggi-do, 17579, Republic of Korea

    Muhammad Zubair Siddiqi & Wan-Taek Im

Authors
  1. Minseok M. Kim
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  2. Muhammad Zubair Siddiqi
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  3. Wan-Taek Im
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Correspondence to Wan-Taek Im.

Additional information

The digital protologue database (DPD) number for the strain Gsoil 3017T is TA00192. The GenBank accession number for the 16S rRNA gene sequence of strain Gsoil 3017T is AB267721.

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Kim, M.M., Siddiqi, M.Z. & Im, WT. Mucilaginibacter ginsenosidivorans sp. nov., Isolated from Soil of Ginseng Field. Curr Microbiol 74, 1382–1388 (2017). https://doi.org/10.1007/s00284-017-1329-4

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  • DOI: https://doi.org/10.1007/s00284-017-1329-4

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