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Flavobacterium qiangtangensis sp. nov., Isolated from Qiangtang Basin in Qinghai-Tibetan Plateau, China

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Abstract

A flexirubin-type yellow-pigmented, non-gliding, non-flagellated, gram-negative bacterium strain, designated F3T, was isolated from a drilling core sample of the Qiangtang basin, Qinghai-Tibetan plateau, China. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain F3T belongs to the genus Flavobacterium, with the highest 16S rRNA gene sequence similarity to the Flavobacterium noncentrifugens CGMCC 1.10076T (94.92 %). Strain F3T grew optimally at temperature about 20 °C, at pH about 7.0–8.0, at NaCl concentration 0 % (w/v). The DNA G+C content of the isolate was 35.5 mol%. The major polar lipid was phosphatidylethanolamine, predominant cellular fatty acids of the strain was iso-C15:0 (22.02 %), while the major menaquinone was menaquinone 6. Due to the phenotypic and genetic distinctiveness and several other characteristic studied in this article, we consider F3T as a novel species of the genus Flavobacterium, and propose to name it Flavobacterium qiangtangensis sp. nov. The type strain is F3T (=CGMCC 1.12706T = JCM 19739T).

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References

  1. Ali Z, Cousin S, Fruhling A et al (2009) Flavobacterium rivuli sp. nov., Flavobacterium subsaxonicum sp. nov., Flavobacterium swingsii sp. nov. and Flavobacterium reichenbachii sp. nov., isolated from a hard water rivulet. Int J Syst Evol Microbiol 59:2610–2617

    Article  CAS  PubMed  Google Scholar 

  2. Aslam Z, Im WT, Kim MK et al (2005) Flavobacterium granuli sp. nov., isolated from granules used in a wastewater treatment plant. Int J Syst Evol Microbiol 55:747–751

    Article  CAS  PubMed  Google Scholar 

  3. Bergey DH, Harrison FC, Breed RS, Hammer BW, Huntoon FME (1923) Bergey’s manual of determinative bacteriology. Williams & Wilkins, Baltimore

    Google Scholar 

  4. Bernardet J-F, Segers P, Vancanneyt M et al (1996) Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46:128–148

    Article  Google Scholar 

  5. Bernardet JF (2002) Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070

    Article  CAS  PubMed  Google Scholar 

  6. Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917

    Article  CAS  PubMed  Google Scholar 

  7. Bowman JP (2000) Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50(5):1861–1868

    CAS  PubMed  Google Scholar 

  8. Chun J, Lee JH, Jung Y et al (2007) EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261

    Article  CAS  PubMed  Google Scholar 

  9. Collins M (1985) Analysis of isoprenoid quinones. Methods Microbiol 18:329–366

    Article  CAS  Google Scholar 

  10. Collins M, Goodfellow M, Minnikin D (1980) Fatty acid, isoprenoid quinone and polar lipid composition in the classification of Curtobacterium and related taxa. J Gen Microbiol 118:29–37

    CAS  PubMed  Google Scholar 

  11. Cowan ST, Steel KJ, Barrow G et al (2004) Cowan and Steel’s manual for the identification of medical bacteria. Cambridge University Press, Cambridge

    Google Scholar 

  12. Dong K, Xu B, Zhu F et al (2013) Flavobacterium hauense sp. nov., isolated from soil and emended descriptions of Flavobacterium subsaxonicum, Flavobacterium beibuense and Flavobacterium rivuli. Int J Syst Evol Microbiol 63:3237–3242

    Article  CAS  PubMed  Google Scholar 

  13. Gerhardt P, Wood WA, Krieg NR (1994) Methods for general and molecular bacteriology. American Society for Microbiology, Washington

    Google Scholar 

  14. Gounot A-M (1986) Psychrophilic and psychrotrophic microorganisms. Experientia 42:1192–1197

    Article  CAS  PubMed  Google Scholar 

  15. Krieg NR, Padgett PJ (2011) Phenotypic and physiological characterization methods. In: Fred R, Aharon O (eds) Methods in microbiology. Academic Press, Waltham, pp 15–60

    Google Scholar 

  16. Leifson E (1951) Staining, shape, and arrangement of bacterial flagella. J Bacteriol 62:377

    CAS  PubMed Central  PubMed  Google Scholar 

  17. Lim CS, Oh YS, Lee JK et al (2011) Flavobacterium chungbukense sp. nov., isolated from soil. Int J Syst Evol Microbiol 61:2734–2739

    Article  CAS  PubMed  Google Scholar 

  18. Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167

    Article  CAS  Google Scholar 

  19. Minnikin D, Collins M, Goodfellow M (1979) Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Microbiol 47:87–95

    CAS  Google Scholar 

  20. Minnikin DE, O’donnell AG, Goodfellow M et al (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241

    Article  CAS  Google Scholar 

  21. Morita RY (1975) Psychrophilic bacteria. Bacteriol Rev 39:144

    CAS  PubMed Central  PubMed  Google Scholar 

  22. Odumeru JA, D’amore T, Russell I et al (1993) Alterations in fatty acid composition and trehalose concentration of saccharomyces brewing strains in response to heat and ethanol shock. J Ind Microbiol 11:113–119

    Article  CAS  Google Scholar 

  23. Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids

  24. Steven B, Briggs G, Mckay CP et al (2007) Characterization of the microbial diversity in a permafrost sample from the Canadian high Arctic using culture-dependent and culture-independent methods. FEMS Microbiol Ecol 59:513–523

    Article  CAS  PubMed  Google Scholar 

  25. Tamura K, Peterson D, Peterson N et al (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  26. Tindall BJ, Rossello-Mora R, Busse HJ et al (2010) Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 60:249–266

    Article  CAS  PubMed  Google Scholar 

  27. Van Trappen S (2003) Flavobacterium gelidilacus sp. nov., isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 53:1241–1245

    Article  PubMed  Google Scholar 

  28. Wilhelm RC, Niederberger TD, Greer C et al (2011) Microbial diversity of active layer and permafrost in an acidic wetland from the Canadian high arctic. Can J Microbiol 57:303–315

    Article  CAS  PubMed  Google Scholar 

  29. Yi H, Oh HM, Lee JH et al (2005) Flavobacterium antarcticum sp. nov., a novel psychrotolerant bacterium isolated from the Antarctic. Int J Syst Evol Microbiol 55:637–641

    Article  CAS  PubMed  Google Scholar 

  30. Yoon JH, Park S, Kang SJ et al (2010) Flavobacterium ponti sp. nov., isolated from seawater. Int J Syst Evol Microbiol 61:81–85

    Article  PubMed  Google Scholar 

  31. Zhang G, Ma X, Niu F et al (2007) Diversity and distribution of alkaliphilic psychrotolerant bacteria in the Qinghai-Tibet Plateau permafrost region. Extremophiles 11:415–424

    Article  CAS  PubMed  Google Scholar 

  32. Zhu L, Liu Q, Liu H et al (2013) Flavobacterium noncentrifugens sp. nov., a psychrotolerant bacterium isolated from glacier meltwater. Int J Syst Evol Microbiol 63:2032–2037

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

The work was supported by the National Special Research Fund (Gas Hydrate Resource Exploration and Production Testing Project. Grant No.: GZHL20110308, GZHL20110323), we thanks the identification service of the China General Microbiological Culture Collection Center (CGMCC) for their help.

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Authors and Affiliations

  1. College of Life Science and Technology, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Chaoyang District, Beijing, 100029, People’s Republic of China

    Faqi Huang, Yali Zhang & Jie Lv

  2. Oil and Gas Survey, China Geological Survey, Beijing, 100029, People’s Republic of China

    Youhai Zhu & Pingkang Wang

  3. School of Applied Sciences, Institute for Applied Ecology New Zealand, Auckland University of Technology, Auckland, New Zealand

    Jun Lu

  4. Faculty of Health and Environmental Sciences, School of Interprofessional Health Studies, Auckland University of Technology, Auckland, New Zealand

    Jun Lu

  5. Institute of Biomedical Technology, Auckland University of Technology, 34 St Paul Street, Auckland, 1142, New Zealand

    Jun Lu

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  1. Faqi Huang
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  2. Yali Zhang
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  3. Youhai Zhu
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  4. Pingkang Wang
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  5. Jun Lu
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Correspondence to Jie Lv.

Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain F3T is KF726983.

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Huang, F., Zhang, Y., Zhu, Y. et al. Flavobacterium qiangtangensis sp. nov., Isolated from Qiangtang Basin in Qinghai-Tibetan Plateau, China. Curr Microbiol 69, 234–239 (2014). https://doi.org/10.1007/s00284-014-0579-7

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  • DOI: https://doi.org/10.1007/s00284-014-0579-7

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