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Bioconversion of 2-cyanopyrazine to 5-hydroxypyrazine-2-carboxylic acid with Agrobacterium sp. DSM 6336

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Abstract

5-Hydroxypyrazine-2-carboxylic acid, a versatile building block for the synthesis of new antituberculous agents, was prepared by whole-cell biotransformation from 2-cyanopyrazine via pyrazinecarboxylic acid using Agrobacterium sp. DSM 6336. By developing a fermentation process for this two-enzyme-step bioconversion, a product concentration of 286 mM (40 g/l) was obtained. After the isolation method had been optimized the total yield was 80%.

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Authors and Affiliations

  1. Biotechnology Research Department, Lonza AG, CH-3930 Visp, Switzerland Tel.: +41 27 9486083; Fax: +41 27 9476083 email: andreas.kiener@Lonia.ch, , , , , , CH

    M. Wieser, K. Heinzmann & A. Kiener

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  1. M. Wieser
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  2. K. Heinzmann
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  3. A. Kiener
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Received: 28 February 1997 / Received revision: 28 April 1997 / Accepted: 4 May 1997

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Wieser, M., Heinzmann, K. & Kiener, A. Bioconversion of 2-cyanopyrazine to 5-hydroxypyrazine-2-carboxylic acid with Agrobacterium sp. DSM 6336. Appl Microbiol Biotechnol 48, 174–176 (1997). https://doi.org/10.1007/s002530051034

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  • DOI: https://doi.org/10.1007/s002530051034

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