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High-cell-density cultivation for co-production of ergosterol and reduced glutathione by Saccharomyces cerevisiae

  • Biotechnological Products and Process Engineering
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Abstract

Two different high-cell-density cultivation processes based on the mutant Saccharomyces cerevisiae GE-2 for simultaneous production of glutathione and ergosterol were investigated. Compared with keeping the ethanol volumetric concentration at a constant low level, feedback control of glucose feeding rate (F) by keeping the descending rate of ethanol volumetric concentration (ΔE/Δt) between −0.1% and 0.15% per hour was much more efficient to achieve a high glutathione and ergosterol productivity. This bioprocess overcomes some disadvantages of traditional S. cerevisiae-based cultivation process, especially shortening cultivation period and making the cultivation process steady-going. A classical on or off controller was used to manipulate F to maintain ΔE/Δt at its set point. The dry cell weight, glutathione yield and ergosterol yield reached 110.0 ± 2.6 g/l, 2,280 ± 76 mg/l, and 1,510 ± 28 mg/l in 32 h, respectively.

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Acknowledgements

We thank the support of Nation Science Foundation of China (20576013), 973 Program (2007CB707804), Beijing Natural Science Foundation (20721002), Beijing Science Program (D0205004040211), and National Science Fund for Distinguished Young Scholars (20325622).

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Authors and Affiliations

  1. Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People’s Republic of China

    Fei Shang, Zheng Wang & Tianwei Tan

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  1. Fei Shang
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  2. Zheng Wang
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  3. Tianwei Tan
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Correspondence to Tianwei Tan.

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Shang, F., Wang, Z. & Tan, T. High-cell-density cultivation for co-production of ergosterol and reduced glutathione by Saccharomyces cerevisiae . Appl Microbiol Biotechnol 77, 1233–1240 (2008). https://doi.org/10.1007/s00253-007-1272-6

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  • DOI: https://doi.org/10.1007/s00253-007-1272-6

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