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Extraction, structural characterization and antioxidant activity of polyhydroxylated 1,4-naphthoquinone pigments from spines of sea urchin Glyptocidaris crenularis and Strongylocentrotus intermedius

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Abstract

In the present study, the effects of operational parameters on adsorption and desorption capacity of polyhydroxylated 1,4-naphthoquinone (PHNQ) pigments on macroporous resin in static mode were evaluated. On this basis, a suitable operating condition was selected which recovered 83.96 ± 0.71 and 80.58 ± 1.48 % of PHNQ pigments from Glyptocidaris crenularis and Strongylocentrotus intermedius spines, respectively. Based on accurate molecular mass and ultraviolet–visible (UV–Vis) spectral data acquired by ultra-performance liquid chromatography hybrid quadrupole orthogonal acceleration time-of-flight mass spectrometer (UPLC Q-TOFMS), spinochrome E, spinochrome D and spinochrome B were tentatively identified in the pigment extract prepared from G. crenularis. In contrast, only spinochrome B was identified in the pigment extract from S. intermedius. Between the two extracts, the one from G. crenularis showed stronger DPPH radical scavenging ability and ferrous ion chelating capacity. In our opinion, the different PHNQ compounds existing in the two extracts resulted in the difference in antioxidant activity.

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Acknowledgments

This work was financially supported by “The National High Technology Research and Development Program of China (863 Program) (No. 2011AA100803)” and “The Research Start-up Project for Doctor Funded by Liaoning Science and Technology Department (No. 20091002)”.

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None.

Compliance with Ethics Requirements

This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. School of Food Science and Technology, Dalian Polytechnic University, Engineering Research Center of Seafood of Ministry of Education, International Science and Technology Cooperation Base for Precious Seafood Deep Processing, Dalian, 116034, People’s Republic of China

    Dong-Mei Li, Da-Yong Zhou, Bei-Wei Zhu, Li Miao, Lei Qin, Xiu-Ping Dong & Xiao-Dong Wang

  2. Department of Biological Resources Chemistry, Faculty of Agriculture, Okayama University, Okayama, 700-8530, Japan

    Yoshiyuki Murata

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  1. Dong-Mei Li
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Correspondence to Bei-Wei Zhu.

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Li, DM., Zhou, DY., Zhu, BW. et al. Extraction, structural characterization and antioxidant activity of polyhydroxylated 1,4-naphthoquinone pigments from spines of sea urchin Glyptocidaris crenularis and Strongylocentrotus intermedius . Eur Food Res Technol 237, 331–339 (2013). https://doi.org/10.1007/s00217-013-1996-8

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  • DOI: https://doi.org/10.1007/s00217-013-1996-8

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