Abstract
Acetaldehyde binds to some proteins and becomes a Schiff base. It is assumed that after the consumption of ethanol the acetaldehyde binds to the proteins to form adducts, and such acetaldehyde adducts are associated with organ diseases. We investigated the detection of acetaldehyde adducts in the brain region of a human alcoholics. Brain samples collected from an alcoholic autopsied case were used. Determination of acetaldehyde adducts was performed using a fluorescence immmunohistochemical staining method with antibodies against acetaldehyde adducts. We demonstrated acetaldehyde adducts in the frontal cortex and the midbrain of an alcoholics. Our studies showed that an acetaldehyde adduct was produced in the brain of alcoholics.
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References
Donohue TM, Tuma DJ, Sorrell MF (1983) Binding of metabolically derived acetaldehyde to hepatic proteins in vitro. Lab Invest 49:226–231
Fang JL, Vaca CE (1997) Detection of DNA adducts of acetaldehyde in peripheral white blood cells of alcohol abusers. Carcinogenesis 18:627–632
Freund G, Ballinger WE (1988) Loss of cholinergic muscarinic receptors in the frontal cortex of alcohol abusers. Alcohol Clin Exp Res 12:630–638
Freund G, Ballinger WE (1989a) Neuroreceptor changes in the putamen of alcohol abusers. Alcohol Clin Exp Res 13:213–218
Freund G, Ballinger WE (1989b) Loss of muscarinic cholinergic receptors from temporal cortex of alcohol abusers. Metab Brain Dis 4:121–141
Hoerner M, Behrens UJ, Worner TM, Blacksberg I, Braly LF, Schaffner F, Lieber CS (1988) The role of alcoholism and liver disease in the appearance of serum antibodies against acetaldehyde adducts. Hepatology 8:569–574
Horner M, Behrens UJ, Worner T, Lieber CS (1996) Humoral immune response to acetaldehyde adducts in alcoholic patients. Res Commun Chem Pathol Pharmacol 54:3–12
Israel Y, Hurwitz E, Niemela O, Arnon R (1986) Monoclonal and polyclonal antibodies against acetaldehyde containing epitopes in acetaldehyde protein adduct. Proc Natl Acad Sci USA 83:7923–7927
Iwahashi K, Itoh M, Nakamura K, Ameno K, Suwaki H, Takeuchi Y (1997) Acetaldehyde adducts in the cerebral cortex of ethanol-fed mice. [in Japanese]. Nihon Arukoru Yakubutsu Igakkai Zasshi 32:489–94
Lieber CS (1994) Alcohol and the liver: 1994 update. Gastroenterology 106:1085–1105
Nakamura K, Iwahashi K, Itoh M, Ameno K, Ijiri I, Takeuch Y, Suwaki H (2000) Immunohistochemical study on acetaldehyde adducts in alcohol-fed mice. Alcohol Clin Exp Res 24:93S–96S
Nakano T, Nakamura K, Suwaki H, Itoh M, Ameno K, Itoh K, Iwahashi K (1999) Acetaldehyde adducts in the cerebral cortex of long-term ethanol-fed mice [in Japanese]. Nihon Arukoru Yakubutsu Igakkai Zasshi 34:549–57
Niemela O, Klajner F, Orrego H, Vidins E, Blendis L, Israel Y (1987) Antibodies against acetaldehyde-modified protein epitopes in human alcoholics. Hepatology 7:1210–1214
Niemela O, Juvonen T, Parkkila S (1991) Immunohistochemical demonstration of acetaldehyde-modified epitopes in human liver after alcohol consumption. J Clin Invest 87:1367–1374
Niemela O, Parkkila S, Yla-Herttuala S, Villanueva J, Ruebner B, Halsted CH (1995) Sequential acetaldehyde production, lipid peroxidation, and fibrogenesis in micropig model of alcohol-induced liver disease. Hepatology 22:1208–1214
Phillips SC (1989) Cytoprotective value of lysine, penicillamine, and pyridoxal phosphate against the neurotoxicity of acetaldehyde. Toxicol Appl Pharmacol 98:553–560
Pietrzak ER, Shanley BC, Kroon PA (1995) Antibodies made against a formaldehyde–protein adduct cross react with an acetaldehyde–protein adduct. Implications for the origin of antibodies in human serum which recognize acetaldehyde–protein adducts. Alcohol Alcohol 30:373–378
Sorrell MF, Tuma DJ (1985) Hypothesis: alcohol liver injury and the covalent binding of acetaldehyde. Alcohol Clin Exp Res 9:306–309
Steinberg JJ, Oliver GW Jr, Cajigas A (1997) The formation and measurement of DNA neuroadduction in alcoholism. Case report. Am J Forensic Med Pathol 18:84–91
Stevens VJ, Fanil WJ, Newman CB, Sims RV, Cerami A, Peterson CM (1981) Acetaldehyde adduct with hemoglobin. J Clin Invest 67:361–369
Worrall S, De Jersey J, Wilce PA, Seppa K, Hurme L, Sillanaukee P (1996) Relationship between alcohol intake and immunoglobulin a immunoreactivity with acetaldehyde-modified bovine serum albumin. Alcohol Clin Exp Res 20:836–840
Yokoyama H (1993) Immune response against protein epitopes modified with acetaldehyde and its clinical significance in alcoholic liver diseases. Jpn J Alcohol Drug Depend 28:483–496
Yokoyama H, Ishii H, Nagata S, Kato S, Kamegawa K, Tsuchiya M (1993) Experimental hepatitis induced by ethanol after immunization with acetaldehyde adducts. Hepatology 17:13–19
Yokoyama H, Nagata S, Moriya S, Kato S, Ito T, Kamagawa K, Ishii H (1995) Hepatic fibrosis produced in guinea pigs by chronic ethanol administration and immunization with acetaldehyde adducts. Hepatology 21:1438–1442
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Nakamura, K., Iwahashi, K., Furukawa, A. et al. Acetaldehyde adducts in the brain of alcoholics. Arch Toxicol 77, 591–593 (2003). https://doi.org/10.1007/s00204-003-0465-8
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DOI: https://doi.org/10.1007/s00204-003-0465-8