Abstract
A novel chemolithoautotrophic bacterium, strain H1576T, was isolated from water of a brackish lake. Strain H1576T grew aerobically on inorganic sulfur compounds. Hydrogen gas did not support autotrophic growth, and heterotrophic growth was not observed. Cells were rod shaped, motile, 1.5–2.7 μm in length and 0.6–0.7 μm in width. Growth was observed at 3–22 °C with an optimum growth temperature of 13–15 °C. The pH range for growth was 6.0–7.4 with an optimum pH of 6.6–6.8. Major fatty acids were summed feature 3 (C16: 1ω7c and/or C16: 1ω6c). The complete genome of strain H1576T consists of a circular chromosome and a plasmid, with total length of 2.8 Mbp and G+C content of 46.4 mol%. Phylogenetic analyses indicated that strain H1576T belongs to the genus Sulfurimonas but distinct from representatives of existing species. On the basis of genomic and phenotypic characteristics, a new species named Sulfurimonas aquatica sp. nov. is proposed with the type strain of strain H1576T (= BCRC 81254T = JCM 35004T).
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Acknowledgements
This study was and supported by JSPS KAKENHI, Grant Number 15K07209. The authors have no relevant financial or non-financial interests to disclose. The water sample collection was performed with permission of the Agency of Cultural Affairs, Government of Japan.
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This study was funded by JSPS KAKENHI, Grant Number 15K07209.
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MF conducted water sampling. HK, YK and TW performed experiments for phenotypic characterization. HK isolated the strain, conducted genome analysis, wrote manuscript and prepared figures. All authors reviewed the manuscript.
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Kojima, H., Kato, Y., Watanabe, T. et al. Sulfurimonas aquatica sp. nov., a sulfur-oxidizing bacterium isolated from water of a brackish lake. Arch Microbiol 204, 559 (2022). https://doi.org/10.1007/s00203-022-03167-3
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DOI: https://doi.org/10.1007/s00203-022-03167-3