Abstract
Two aerobic, Gram-stain variable, catalase-positive and oxidase-negative rods named strain UniB2T and UniB3T, were isolated from digestive syrup containing fungal diastase (10 mg/ml), pepsin (2 mg/ml) and sugar base containing polyethylene glycol. Based on 16S rRNA gene sequence analysis, strain UniB2T has the highest sequence similarity with Paenibacillus humicus NBRC 102415T (98.3%) and strain UniB3T showed the highest sequence similarity with Niallia circulans DSM 11T (98.9%). The DNA G + C content of UniB2T was 63.7 mol %. The dDDH and ANI values between the strain UniB2T and its phylogenetically close relative were < 38.3% and < 89.5%, respectively. The major fatty acids of the strain UniB2T were C16:0 (13.9%), C15:0 anteiso (39.7%), C17:0 anteiso (15.5%). The DNA G + C content of UniB3T was 35.6 mol %. The dDDH and ANI values between the strain UniB3T and its close relatives were < 29.1% and 84.6%, respectively. The major fatty acids of strain UniB3T were C16:0 (13.5%), C15:0 anteiso (40.1%) and C17:0 anteiso (16.0%). Major polar lipids for both strains were Diphosphatidylglycerol and phosphatidylethanolamine. Both strains showed unique carbon utilization and assimilation pattern that differentiated them from their phylogenetically related neighbours. These phenotypic, genotypic and chemotaxonomic characters indicated the strains UniB2T and UniB3T represent two novel species for which the names Paenibacillus albicereus sp. nov. (Type strain UniB2T = MCC 3997T = KCTC 43095T = JCM34513T) and Niallia alba sp. nov. (Type strain UniB3T = MCC 3998T = KCTC 43235T = JCM 34492T) are proposed.
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Data availability
The GenBank/EMBL/DDBJ accession numbers for the reference 16S rRNA gene sequences of the strain UniB2T and UniB3T are MK751590 and MK751589, respectively. The accession numbers of whole genomes of UniB2T and UniB3T are CP051428 and JABBPK000000000, respectively.
Change history
31 March 2023
A Correction to this paper has been published: https://doi.org/10.1007/s00203-023-03509-9
Abbreviations
- OrthoANI:
-
Orthologous average nucleotide identity
- dDDH:
-
Digital DNA–DNA hybridization
- API:
-
Analytical profile index
- OGRI:
-
Overall genome relatedness index
- DPG:
-
Diphosphatidylglycerol
- PE:
-
Phosphatidylethanolamine
- PG:
-
Phosphatidylglycerol
- PL:
-
Unknown phospholipid lipid
- APL:
-
Unknown amino phospholipid
- L:
-
Unknown lipid
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Funding
Authors acknowledge the project funding and fellowships to BT by the National Medicinal Plant Board (NMPB), Ministry of Ayush, Government of India under grant number Z.18017/187/CSS/R&D/KR-03/2019-20-NMPB-IV A; authors also acknowledge the funding by the Department of Biotechnology (DBT), Government of India under grant number BT/COORD.II/01/03/2016 (NCMR) used for in-house laboratory facilities. Authors gratefully acknowledge the University Grant Commission (UGC) of the Government of India for providing CSIR-UGC NET-JRF fellowship to KK (Ref. No. 857/CSIR-UGC NET JUNE 2017).
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VT, KK, PD and PK carried out the polyphasic taxonomy experiments; VT, KK, and BT did the phenotypic and genome data analysis and wrote the first draft of the manuscript. YS and SS provided the project funding and in house facilities. TL and AY did the formal analysis, reviewed, edited and finalized the manuscript. AY conceptualized, lead the investigation and provided the funding for the study. All the authors reviewed and approved the final version of the paper.
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Communicated by Erko Stackebrandt.
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203_2021_2749_MOESM1_ESM.pptx
Supplementary file1 (PPTX 4869 KB) Figure S1. Scanning Electron Micrograph of strain UniB2T (a) and UniB3T (b). Figure S2. Polar Lipid Analysis of strain UniB2T and its closest phylogenetic neighbours. Paenibacillus humicus (NBRC 102415T), Paenibacillus pasadenensis NBRC 101214T(a); Polar Lipid Analysis of strain UniB3T and its closest phylogenetic neighbours. Niallia circulans DSM 11T and Niallia nealsonii DSM 15077T (b). All the data obtained from this study. Figure S3. COG functional category distribution of genes in Paenibacillus albicereus UniB2T, Niallia alba UniB3T and their closely related type strains
203_2021_2749_MOESM2_ESM.docx
Supplementary file2 (DOCX 31 KB) Table S1. List of all traits which are not included in differential phenotypic characteristics Table 1 of Strain UniB2T and its closest relatives. Table S2. List of all traits which are not included in differential phenotypic characteristics Table 2 of Strain UniB3T and its closest relatives. Table S3. Cellular fatty acid content (%) of strain and its closest phylogenetic neighbours. Paenibacillus humicus (NBRC 102415T), P. pasadenensis NBRC 101214T. Table S4. Cellular fatty acid content (%) of strain and its closest phylogenetic neighbours. Niallia circulans DSM 11T and N. nealsonii DSM 15077T. Table S5. Genome statistics of Paenibacillus albicereus UniB2T (CP051428), Niallia alba UniB3T (JABBPK000000000) and their taxonomically closest relatives.
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Thorat, V., Kirdat, K., Tiwarekar, B. et al. Paenibacillus albicereus sp. nov. and Niallia alba sp. nov., isolated from digestive syrup. Arch Microbiol 204, 127 (2022). https://doi.org/10.1007/s00203-021-02749-x
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DOI: https://doi.org/10.1007/s00203-021-02749-x