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Muricauda chongwuensis sp. nov., isolated from coastal seawater of China

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Abstract

In the course of screening for bacterial predators, a Gram-stain-negative, non-flagellated, gliding, long rod-shaped, and yellow-pigmented bacterium, designated strain HICWT, was isolated from coastal seawater of China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HICWT represented a member of the genus Muricauda and showed the highest sequence similarity to M. aquimarina JCM11811T (98.8%) and M. ruestringensis DSM13258T (98.1%). The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain HICWT and M. aquimarina JCM11811T were 79.2% and 34.1%, respectively. NaCl was required for growth. Optimum growth occurred at 25–30 °C, 2.0–3.0% (w/v) NaCl with pH 7.0. Strain HICWT showed some similar characteristics to the nonobligate bacterial predators, and the cells can attach to the prey cells. Strain HICWT contained MK-6 as the predominant respiratory quinone and had iso-C15:0, iso-C15:1 G, and iso-C17:0 3-OH as the major cellular fatty acids. The polar lipids contained phosphatidylethanolamine (PE), three unidentified phospholipids (PL1–PL3), one unidentified amino lipids (AL), and three unidentified polar lipids (L1–L3). The genome size of strain HICWT was approximately 3.8 Mbp, with a G + C content of 41.4%. Based on the polyphasic evidence, strain HICWT is proposed as representing a new species of the genus Muricauda, for which the name Muricauda chongwuensis sp. nov. is proposed. The type strain is HICWT (= JCM 33643 T = MCCC 1K03769T).

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Data availability

The GenBank/EMBL/DDBJ accession numbers for the draft genome sequence and the 16S rRNA gene sequence of Muricauda chongwuensis HICWT are WYET00000000 and MK920190, respectively.

Code availability

Not applicable.

Abbreviations

BALOs:

Bdellovibrio-and-like organisms

rRNA:

Ribosomal RNA

MB:

Marine broth 2216E

MA:

Marine agar 2216E

MCCC:

Marine Culture Collection of China

JCM:

Japan Collection of Microorganisms

TEM:

Transmission electron microscope

gANI:

Genome average nucleotide identity

dDDH:

Digital DNA–DNA hybridization

MP:

Maximum parsimony

ML:

Maximum likelihood

NJ:

Neighbor joining

OMVs:

Outer membrane vesicles

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Funding

This work was supported by the National Natural Science Foundation of China (Grant no. 41506179), National Programme on Global Change and Air-Sea Interaction (Grant no. GASI-03-01-03-01), Natural Science Foundation of Fujian Province (Grant no. 2015J01613), and Fujian Provincial Key Laboratory of Marine Ecological Conservation and Restoration (Grant no. EPR2020002).

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Authors and Affiliations

  1. College of Chemical Engineering, Huaqiao University, 668 Jimei Road, Xiamen, 361021, Fujian, People’s Republic of China

    Ming-Xia Chen & Xiao-Yu He

  2. Third Institute of Oceanography, Ministry of Natural Resources, 178 Daxue Road, Xiamen, 361005, Fujian, People’s Republic of China

    He-Yang Li

  3. Fujian Provincial Key Laboratory of Marine Ecological Conservation and Restoration, Xiamen, 361005, Fujian, People’s Republic of China

    He-Yang Li

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  1. Ming-Xia Chen
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Contributions

M-XC and H-YL conceived the project. M-XC and X-YH experimented and analyzed the data. M-XC wrote the manuscript. All authors have revised the manuscript.

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Correspondence to Ming-Xia Chen or He-Yang Li.

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Communicated by Erko Stackebrandt.

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Chen, MX., He, XY. & Li, HY. Muricauda chongwuensis sp. nov., isolated from coastal seawater of China. Arch Microbiol 203, 6245–6252 (2021). https://doi.org/10.1007/s00203-021-02591-1

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