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Methylobrevis albus sp. nov., isolated from freshwater lake sediment

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Abstract

An aerobic, Gram-stain-negative, rod-shaped bacterium with flagellum, designated L22T, was isolated from sediment of Hulun Lake, Inner Mongolia, China. The organism was found to grow optimally at 30° C in a medium containing 0–0.75% (w/v) NaCl at pH 7.5. The major fatty acid identified was summed feature 8 (C18:1ω7c). The dominant polar lipids were phosphomonoester, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The main respiratory quinone was Q-10. The draft genome sequence of strain L22T consisted of 4354,788 bp. The G + C content of genomic DNA was 69.8 mol %. The 16S rRNA gene sequences indicated that strain L22T was affiliated with the genus Methylobrevis within the family Pleomorphomonadaceae, being most closely related to Methylobrevis pamukkalensis JCM 30229T with 95.9% 16S rRNA gene sequences similarity. The AAI, ANI and dDDH values between strain L22T and M. pamukkalensis JCM 30229T were 72.5%, 80.7% and 22.7%. Based on taxonomic results in this study, we proposed that strain L22T a novel species in the genus Methylobrevis of the family Pleomorphomonadaceae, for which the name Methylobrevis albus sp. nov. is proposed. The type strain is L22T (=KCTC 72858T=MCCC 1H00432T).

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Abbreviations

KCTC :

Korean Collection for Type Cultures

MCCC :

Marine Culture Collection of China

MEGA :

Molecular Evolutionary Genetics Analysis

HPLC :

High-performance liquid chromatography

TLC :

Thin-layer chromatography

JCM :

Japan Collection of Microorganisms

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Funding

This work was supported by the National Natural Science Foundation of China (Grant No. 32070002 and Grant No. 31770002) and the National Science and Technology Fundamental Resources Investigation Program of China (Grant No. 2019FY100700).

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Authors and Affiliations

  1. Marine College, Shandong University, Weihai, 264209, People’s Republic of China

    Qi-Hang Zou, Xi Feng, Tao-Jie Wang & Zong-Jun Du

  2. State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, People’s Republic of China

    Zong-Jun Du

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  1. Qi-Hang Zou
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  2. Xi Feng
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  3. Tao-Jie Wang
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  4. Zong-Jun Du
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Correspondence to Zong-Jun Du.

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Communicated by Erko Stackebrandt.

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The GenBank accession number for the 16S rRNA gene sequence of Methylobrevis albus L22T is MW195049 and the draft genome has been deposited in GenBank under the accession number JADZLT000000000.

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Zou, QH., Feng, X., Wang, TJ. et al. Methylobrevis albus sp. nov., isolated from freshwater lake sediment. Arch Microbiol 203, 4549–4556 (2021). https://doi.org/10.1007/s00203-021-02442-z

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