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Description of Gemmobacter aestuarii sp. nov., isolated from estuarine surface water and reclassification of Cereibacter changlensis as Gemmobacter changlensis Chen et al. 2013

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Abstract

A Gram-stain-negative, tyrosine-metabolizing, non-motile, strictly aerobic, non-spore-forming, rod-shaped marine bacterium, designated strain CC-PW-75T, was isolated from the estuarine water off Pintung, Taiwan. Strain CC-PW-75T formed a distinct phyletic lineage associated with Gemmobacter species, sharing the highest 16S rRNA gene sequence similarity with G. megaterium CF17T and G. straminiformis CAM-8T (96.0% each) followed by G. aquatilis IFAM 1031T and G. nectariphilus AST4T (95.8% each). Analysis of the draft genome (3.76 Mbp) revealed the presence of genes encoding light-harvesting complexes, photosynthetic reaction centers and proteins involved in the metabolism of CO, CO2, HCO3‒ and H2S. However, bacteriochlorophyll a was not detected. Average nucleotide identity values between the genome sequence of CC-PW-75T and the related Gemmobacter species (n = 6) were estimated to be 72.8–76.3%. Polar lipid analysis revealed the presence of phosphatidylglycerol, phosphatidylethanolamine and an unidentified lipid in major amounts, and phosphatidylmonomethylethanolamine, phosphatidylcholine, an unidentified aminolipid and an unidentified lipid in minor amounts. C18:1ω7c and/or C18:1ω6c, C18:0 and C18:1ω7c 11-methyl were identified to be major fatty acids. The DNA G + C content was 66.2 mol% (draft genome sequence). Ubiquinone-10 (Q-10) was the sole respiratory quinone. Based on the polyphasic taxonomic evidence, CC-PW-75T is most likely a novel species of the genus Gemmobacter, affiliated to the family Rhodobacteraceae, for which the name Gemmobacter aestuarii sp. nov. is proposed. The type strain is CC-PW-75T (= JCM 19754T = BCRC 80759T). Also, we propose the reclassification of Cereibacter changlensis as Gemmobacter changlensis Chen et al. 2013 using the polyphasic data presented in this study.

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Acknowledgements

This work was financially supported by the “Innovation and Development Center of Sustainable Agriculture” from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE) (Grant No. 100S0506) in Taiwan.

The GenBank accession number for the 16S rRNA gene sequence of strain CC-PW-75T is KF732818. The draft genome of CC-PW-75T has been deposited at DDBJ/ENA/GenBank under the Accession No. SSND00000000. The version described in this paper is version SSND01000000.

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Authors and Affiliations

  1. Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, 402, Taiwan

    Asif Hameed, Mariyam Shahina, Shih-Yao Lin, Yi-Han Hsu, Wei-An Lai & Chiu-Chung Young

  2. Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City, 811, Taiwan

    Wen-Ming Chen

  3. Innovation and Development Center of Sustainable Agriculture, National Chung Hsing University, Taichung, 402, Taiwan

    Chiu-Chung Young

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  1. Asif Hameed
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Contributions

MS isolated the strain and carried out physiological tests and literature survey. SYL identified the strain and performed the fatty acid analysis. WAL conducted the biochemical analysis. AH performed polar lipid, quinone, and other chemotaxonomic analysis. YHH dealt with hydrolysis of macromolecules and physiological tests in part. AH together with WMC and CCY analyzed the raw data and drafted the manuscript.

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Correspondence to Chiu-Chung Young.

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Communicated by Erko Stackebrandt.

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Hameed, A., Shahina, M., Lin, SY. et al. Description of Gemmobacter aestuarii sp. nov., isolated from estuarine surface water and reclassification of Cereibacter changlensis as Gemmobacter changlensis Chen et al. 2013. Arch Microbiol 202, 1035–1042 (2020). https://doi.org/10.1007/s00203-020-01809-y

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