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Rhodobacter alkalitolerans sp. nov., isolated from an alkaline brown pond

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Abstract

An alkali-tolerant, Gram-stain-negative, motile, rod-to-oval-shaped, yellowish brown-colored, phototrophic bacterium, designated as strain JA916T, was isolated from an alkaline brown pond in Gujarat, India. The DNA G + C content of the strain JA916T was 65.1 mol%. Strain JA916T grew well at pH 10. Respiratory quinone was Q-10 and major fatty acid was C18:1ω7c/C18:1ω6c, with significant quantities of C15:02OH observed. Strain JA916T shared the highest 16S rRNA gene sequence similarity with the type strains of Rhodobacter johrii (98.4%), followed by Rhodobacter megalophilus (98.3%), Rhodobacter sphaeroides (98.3%), Rhodobacter azotoformans (97.9%) and other members of the genus Rhodobacter (< 97%). 16S rRNA gene-based phylogenetic tree shows that strain JA916T formed a distinct sub-clade with Rhodobacter johrii, Rhodobacter megalophilus, Rhodobacter sphaeroides and Rhodobacter azotoformans. Further, rpoB-based phylogenetic analysis showed lower similarity with closely related species (≤ 93.0%) of the genus Rhodobacter, which suggests that JA916T is a novel species of the genus Rhodobacter. DNA–DNA hybridization values between strain JA916T and related type strains were less than 40%. Phenotypic, chemotaxonomical and phylogenetic differences showed that strain JA916T was distinct from other species of the genus Rhodobacter, suggesting strain JA916T represents a new species of the genus for which the name Rhodobacter alkalitolerans sp. nov. is proposed. Type strain is JA916T (= KCTC 15473T = LMG 28749T).

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Acknowledgements

G.S thanks the UGC, New Delhi, for the award of senior research fellowship. Ramana thanks the Department of Biotechnology, Government of India, for the award of Tata Innovative Fellowship. The Department of Science and Technology and University Grants Commission are acknowledged for providing infrastructural facilities through FIST and SAP (DRS). The financial assistance received from the Ministry of Earth Sciences (MoES), Government of India, is acknowledged.

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Author notes

  1. Tushar Lodha

    Present address: National Centre for Microbial Resource, National Centre for Cell Science, Pune, Maharashtra, 411021, India

Authors and Affiliations

  1. Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad, 500 046, India

    Suresh Gandham, Tushar Lodha & Venkata Ramana Chintalapati

  2. Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J. N. T. University, Kukatpally, Hyderabad, 500085, India

    Sasikala Chintalapati

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  1. Suresh Gandham
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  2. Tushar Lodha
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Corresponding authors

Correspondence to Sasikala Chintalapati or Venkata Ramana Chintalapati.

Additional information

Communicated by Erko Stackebrandt.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA and rpoB gene sequences of the strains JA916T are LN810645 and LT630399, respectively. DPD Taxon Number of the strain is TA00378.

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Gandham, S., Lodha, T., Chintalapati, S. et al. Rhodobacter alkalitolerans sp. nov., isolated from an alkaline brown pond. Arch Microbiol 200, 1487–1492 (2018). https://doi.org/10.1007/s00203-018-1561-8

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  • DOI: https://doi.org/10.1007/s00203-018-1561-8

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