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Development of a set of PCR markers specific to Aegilops longissima chromosome arms and application in breeding a translocation line

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Abstract

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Transcriptome data were used to develop 134 Aegilops longissima specific PCR markers and their comparative maps were constructed by contrasting with the homologous genes in the wheat B genome. Three wheat– Ae. longissima 1BL·1S l S translocation lines were identified using the correspondence markers.

Abstract

Aegilops longissima is an important wild species of common wheat that harbors many genes that can be used to improve various traits of common wheat (Triticum aestivum L.). To efficiently transfer the traits conferred by these Ae. longissima genes into wheat, we sequenced the whole expression transcript of Ae. longissima. Using the transcriptome data, we developed 134 specific polymerase chain reaction markers located on the 14 chromosome arms of Ae. longissima. These novel molecular markers were assigned to specific chromosome locations based on a comparison with the homologous genes in the B genome of wheat. Annotation of these genes showed that most had functions related to metabolic processes, hydrolase activity, or catalytic activity. Additionally, we used these markers to identify three wheat–Ae. longissima 1BL·1SlS translocation lines in somatic variation populations resulting from a cross between wheat cultivar Westonia and a wheat–Ae. longissima substitution line 1Sl(1B). The translocation lines had several low molecular weight glutenin subunits encoding genes beneficial to flour processing quality that came from Ae. longissima 1SlS. The three translocation lines were also confirmed by genomic in situ hybridization. These translocation lines will be further evaluated for potential quality improvement of bread-making properties of wheat.

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Acknowledgements

We thank Prof. Yueming Yan at Capital Normal University, China, for providing Ae. longissima accession PI542196 and wheat–Ae. longissima substitution line CB-SLB, Prof. Wenxuan Liu at Henan Agricultural University, China, for providing a set of wheat–Ae. longissima disomic chromosome addition lines, Prof. Wujun Ma at Murdoch University, Australia, for providing wheat cultivar Westonia. We are appreciated to Prof. Hongjie Li at Institute of Crop Science, Chinese Academy of Agricultural Sciences, China, for his critical reading of this manuscript.

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  1. Kunyang Wang, Zhishan Lin and Long Wang contributed equally to this work.

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  1. National Key Facility of Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, People’s Republic of China

    Kunyang Wang, Zhishan Lin, Ke Wang, Lipu Du & Xingguo Ye

  2. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of China

    Long Wang & Qinghua Shi

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  1. Kunyang Wang
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Correspondence to Xingguo Ye.

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This research was financially supported by the National Key Research and Development Program of China (2016YFD0102001 and 2016YFD0102002), the National Natural Science Foundation of China (31771788), and the Agricultural Science and Technology Innovation Program (ASTIP) of Chinese Academy of Agricultural Sciences.

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Communicated by Susanne Dreisigacker.

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Wang, K., Lin, Z., Wang, L. et al. Development of a set of PCR markers specific to Aegilops longissima chromosome arms and application in breeding a translocation line. Theor Appl Genet 131, 13–25 (2018). https://doi.org/10.1007/s00122-017-2982-5

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