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Genetic mapping of a major dominant gene for resistance to Ralstonia solanacearum in eggplant

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Abstract

Resistance of eggplant against Ralstonia solanacearum phylotype I strains was assessed in a F6 population of recombinant inbred lines (RILs) derived from a intra-specific cross between S. melongena MM738 (susceptible) and AG91-25 (resistant). Resistance traits were determined as disease score, percentage of wilted plants, and stem-based bacterial colonization index, as assessed in greenhouse experiments conducted in Réunion Island, France. The AG91-25 resistance was highly efficient toward strains CMR134, PSS366 and GMI1000, but only partial toward the highly virulent strain PSS4. The partial resistance found against PSS4 was overcome under high inoculation pressure, with heritability estimates from 0.28 to 0.53, depending on the traits and season. A genetic map was built with 119 AFLP, SSR and SRAP markers positioned on 18 linkage groups (LG), for a total length of 884 cM, and used for quantitative trait loci (QTL) analysis. A major dominant gene, named ERs1, controlled the resistance to strains CMR134, PSS366, and GMI1000. Against strain PSS4, this gene was not detected, but a significant QTL involved in delay of disease progress was detected on another LG. The possible use of the major resistance gene ERs1 in marker-assisted selection and the prospects offered for academic studies of a possible gene for gene system controlling resistance to bacterial wilt in solanaceous plants are discussed.

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Acknowledgments

The authors are grateful to A. Palloix, M. Pitrat, M. Causse, and V. Lefebvre (Institut National de la Recherche Agronomique (INRA), UR 1052) for their help in analysing the segregation results as well as for early review of this paper. We also thank E. Chapier-Fontaine, J. J. Chéron, J. M. Baptiste and S. Lebon (Centre de coopération International en Recherche Agronomique pour le Développement (CIRAD), Réunion) for providing technical assistance. This work was funded by De Ruiter Seeds, Enza Zaden, Gautier Semences, Nunhems, Rijk Zwaan, and Vilmorin & Cie; the European Regional Development Funds (FEDER) of the European Union, Conseil Régional de la Réunion also provided financial support as part of the programme “Lutte génétique contre les maladies émergentes chez les solanées maraîchères”(GENETOM).

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  1. CIRAD, UMR Peuplements végétaux et Bioagresseurs en Milieu Tropical (PVBMT), 7 chemin de l’IRAT, 97410, Saint Pierre, La Réunion, France

    A. Lebeau, M. Gouy, E. Wicker, F. Chiroleu & J. Dintinger

  2. INRA/CIRAD, UMR PVBMT, 7 Chemin de l’IRAT, 97410, Saint-Pierre Cedex, La Réunion, France

    P. Prior

  3. Unité de Génétique et Amélioration des Fruits et Légumes, Institut National de la Recherche Agronomique (INRA), Centre d’Avignon, UR1052, Domaine St Maurice, BP94, 84143, Montfavet, France

    M. C. Daunay

  4. Department of Molecular Biology and Genetics, Izmir Institute of Technology, Urla, Izmir, 35430, Turkey

    A. Frary

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  1. A. Lebeau
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Lebeau, A., Gouy, M., Daunay, M.C. et al. Genetic mapping of a major dominant gene for resistance to Ralstonia solanacearum in eggplant. Theor Appl Genet 126, 143–158 (2013). https://doi.org/10.1007/s00122-012-1969-5

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