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Dissection of the oligogenic resistance to Cucumber mosaic virus in the melon accession PI 161375

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Abstract

Resistance to Cucumber mosaic virus (CMV) in the exotic melon accession PI 161375, cultivar “Sonwang Charmi” (SC) had previously been described as oligogenic, recessive and quantitative, with a major QTL residing in linkage group XII (LGXII). We have used a collection of near isogenic lines (NILs) with introgressions of SC into the genome of the susceptible accession Piel de Sapo (PS) to further characterise this resistance. Infection of NILs carrying introgressions on LGXII showed that only NIL SC12-1 was resistant to CMV strains P9 and P104.82, but not to strains M6 and TL. Further mapping of this region showed that the resistance, named cmv1 maps in an area of 2.2 cM, between markers CMN61_44 and CMN21_55. Moreover, cmv1 confers total resistance to strains P9 and P104.82, indicating that in these cases it is not quantitative and that cmv1 is sufficient to confer full resistance to these CMV strains. Candidate gene mapping of ten translation initiation factors in the melon genome failed to find any of them in the interval between markers CMN61_44 and CMN21_55. All these results suggest that the resistance to CMV present in SC is oligogenic, where different loci confer resistance to different CMV strains, but not necessarily quantitative, since at least one of these genes (cmv1) confers total resistance, similar to that of the parental SC, and does not need the contribution of other loci.

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References

  • Albar L, Bangratz-Reyser M, Hebrard E, Ndjiondjop MN, Jones M, Ghesquiere A (2006) Mutations in the eIF(iso)4G translation initiation factor confer high resistance of rice to Rice yellow mottle virus. Plant J 47:417–426

    Article  PubMed  CAS  Google Scholar 

  • Caranta C, Pflieger S, Lefebvre V, Daubeze AM, Thabuis A, Palloix A (2002) QTLs involved in the restriction of Cucumber mosaic virus (CMV) long-distance movement in pepper. Theor Appl Genet 104:586–591

    Article  PubMed  CAS  Google Scholar 

  • Deleu W, Gonzalez V, Monfort A, Bendahmane A, Puigdomenech P, Arus P, Garcia-Mas J (2007) Structure of two melon regions reveals high microsynteny with sequenced plant species. Mol Genet Genomics 278:611–622

    Article  PubMed  CAS  Google Scholar 

  • Diaz JA, Mallor C, Soria C, Camero R, Garzo E, Fereres A, Alvarez JM, Gómez-Guillamón ML, Luis-Arteaga M, Moriones E (2003) Potential sources of resistance for melon to nonpersistently aphid-borne viruses. Plant Dis 87:960–964

    Article  Google Scholar 

  • Dogimont C, Leconte L, Perin C, Thabuis A, Lecoq H, Pitrat M (2000) Identification of QTLs contributing to resistance to different strains of Cucumber Mosaic cucumovirus in melon. Acta Hortic 510:391–398

    CAS  Google Scholar 

  • Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15

    Google Scholar 

  • Eduardo I, Arus P, Monforte AJ (2005) Development of a genomic library of near isogenic lines (NILs) in melon (Cucumis melo L.) from the exotic accession PI161375. Theor Appl Genet 112:139–148

    Article  PubMed  CAS  Google Scholar 

  • Frantz JD, Jahn MM (2004) Five independent loci each control monogenic resistance to gummy stem blight in melon (Cucumis melo L.). Theor Appl Genet 108:1033–1038

    Article  PubMed  CAS  Google Scholar 

  • Fraser R (1990) The genetics of resistance to plant viruses. Annu Rev Phytopathol 28:179–200

    Article  Google Scholar 

  • Fukino N, Sakata Y, Kunihisa M, Matsumoto S (2007) Characterisation of novel simple sequence repeat (SSR) markers for melon (Cucumis melo L.) and their use for genotype identification. J Hortic Sci Biotechnol 82:330–334

    CAS  Google Scholar 

  • Gao Z, Johansen E, Eyers S, Thomas CL, Noel Ellis TH, Maule AJ (2004) The potyvirus recessive resistance gene, sbm1, identifies a novel role for translation initiation factor eIF4E in cell-to-cell trafficking. Plant J 40:376–385

    Article  PubMed  CAS  Google Scholar 

  • Garcia-Mas J, Oliver M, Gómez-Paniagua H, de Vicente MC (2000) Comparing AFLP, RAPD and RFLP markers for measuring genetic diversity in melon. Theor Appl Genet 101:860–864

    Article  CAS  Google Scholar 

  • Gonzalez-Ibeas D, Blanca J, Roig C, González-To M, Picó B, Truniger V, Gómez P, Deleu W, Caño-Delgado A, Arús P, Nuez F, Garcia-Mas J, Puigdomènech P, Aranda MA (2007) MELOGEN: an EST database for melon functional genomics. BMC Genomics 8:306

    Article  PubMed  CAS  Google Scholar 

  • Gonzalo MJ, Oliver M, Garcia-Mas J, Monfort A, Dolcet-Sanjuan R, Katzir N, Arus P, Monforte AJ (2005) Simple-sequence repeat markers used in merging linkage maps of melon (Cucumis melo L.). Theor Appl Genet 110:802–811

    Article  PubMed  CAS  Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  • Howad W, Yamamoto T, Dirlewanger E, Testolin R, Cosson P, Cipriani G, Monforte AJ, Georgi L, Abbott AG, Arus P (2005) Mapping with a few plants: using selective mapping for microsatellite saturation of the Prunus reference map. Genetics 171:1305–1309

    Article  PubMed  CAS  Google Scholar 

  • Karchi Z, Cohen S, Govers A (1975) Inheritance of resistance to Cucumber mosaic virus in melons. Phytopathology 65:479–481

    Article  Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  PubMed  CAS  Google Scholar 

  • Lellis AD, Kasschau KD, Whitham SA, Carrington JC (2002) Loss-of-susceptibility mutants of Arabidopsis thaliana reveal an essential role for eIF(iso)4E during potyvirus infection. Curr Biol 12:1046–1051

    Article  PubMed  CAS  Google Scholar 

  • Leroux JP, Quiot JB, Lecoq H, Pitrat M (1979) Mise en évidence et répartition dans le Sud-Est de la France d’un pathotype particulier du Virus de la Mosaïque du Cocombre. Ann Phytopathol 11:431–438

    Google Scholar 

  • Maule AJ, Caranta C, Boulton MI (2007) Sources of natural resistance to plant viruses: status and prospects. Mol Plant Pathol 8:223–233

    Article  CAS  PubMed  Google Scholar 

  • Morales M, Roig E, Monforte AJ, Arus P, Garcia-Mas J (2004) Single-nucleotide polymorphisms detected in expressed sequence tags of melon (Cucumis melo L.). Genome 47:352–360

    Article  PubMed  CAS  Google Scholar 

  • Morales M, Orjeda G, Nieto C, van Leeuwen H, Monfort A, Charpentier M, Caboche M, Arus P, Puigdomenech P, Aranda MA, Dogimont C, Bendahmane A, Garcia-Mas J (2005) A physical map covering the nsv locus that confers resistance to Melon necrotic spot virus in melon (Cucumis melo L.). Theor Appl Genet 111:914–922

    Article  PubMed  CAS  Google Scholar 

  • Moreno E, Obando JM, Dos-Santos N, Fernández-Trujillo JP, Monforte AJ, Garcia-Mas J (2007) Candidate genes and QTLs for fruit ripening and softening in melon. Theor Appl Genet 116:589–602

    Article  PubMed  CAS  Google Scholar 

  • Nieto C, Morales M, Orjeda G, Clepet C, Monfort A, Sturbois B, Puigdomenech P, Pitrat M, Caboche M, Dogimont C, Garcia-Mas J, Aranda MA, Bendahmane A (2006) An eIF4E allele confers resistance to an uncapped and non-polyadenylated RNA virus in melon. Plant J 48:452–462

    Article  PubMed  CAS  Google Scholar 

  • Noueiry AO, Chen J, Ahlquist P (2000) A mutant allele of essential, general translation initiation factor DED1 selectively inhibits translation of a viral mRNA. Proc Natl Acad Sci USA 97:12985–12990

    Article  PubMed  CAS  Google Scholar 

  • Palukaitis P, Roossinck MJ, Dietzgen RG, Francki RIB (1992) Cucumber mosaic virus. Adv Virus Res 41:281–348

    Article  PubMed  CAS  Google Scholar 

  • Perin C, Hagen S, De Conto V, Katzir N, Danin-Poleg Y, Portnoy V, Baudracco-Arnas S, Chadoeuf J, Dogimont C, Pitrat M (2002) A reference map of Cucumis melo based on two recombinant inbred line populations. Theor Appl Genet 104:1017–1034

    Article  PubMed  CAS  Google Scholar 

  • Provvidenti R, Hampton RO (1992) Sources of resistance to viruses in the Potyviridae. Arch Virol Suppl 5:189–211

    PubMed  CAS  Google Scholar 

  • Requena A, Simon-Buela L, Salcedo G, Garcia-Arenal F (2006) Potential involvement of a cucumber homolog of phloem protein 1 in the long-distance movement of Cucumber mosaic virus particles. Mol Plant Microbe Interact 19:734–746

    Article  PubMed  CAS  Google Scholar 

  • Risser G, Pitrat M, Rode JC (1977) Etude de la résistance du melon (Cucumis melo L.) au virus de la mosaïque du concombre. Ann Amél Plant 27:509–522

    Google Scholar 

  • Robaglia C, Caranta C (2006) Translation initiation factors: a weak link in plant RNA virus infection. Trends Plant Sci 11:40–45

    Article  PubMed  CAS  Google Scholar 

  • Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132:365–386

    PubMed  CAS  Google Scholar 

  • Ruffel S, Gallois JL, Moury B, Robaglia C, Palloix A, Caranta C (2006) Simultaneous mutations in translation initiation factors eIF4E and eIF(iso)4E are required to prevent pepper veinal mottle virus infection of pepper. J Gen Virol 87:2089–2098

    Article  PubMed  CAS  Google Scholar 

  • Rusholme RL, Higgins EE, Walsh JA, Lydiate DJ (2007) Genetic control of broad-spectrum resistance to turnip mosaic virus in Brassica rapa (Chinese cabbage). J Gen Virol 88:3177–3186

    Article  PubMed  CAS  Google Scholar 

  • Seo YS, Rojas MR, Lee JY, Lee SW, Jeon JS, Ronald P, Lucas WJ, Gilbertson RL (2006) A viral resistance gene from common bean functions across plant families and is up-regulated in a non-virus-specific manner. Proc Natl Acad Sci USA 103:11856–11861

    Article  PubMed  CAS  Google Scholar 

  • Stamova BS, Chatelat RT (2000) Inheritance and genetic mapping of Cucumber mosaic virus resistance introgressed from Lycopersicon chilense into tomato. Theor Appl Genet 1001:527–537

    Article  Google Scholar 

  • Stein N, Perovic D, Kumlehn J, Pellio B, Stracke S, Streng S, Ordon F, Graner A (2005) The eukaryotic translation initiation factor 4E confers multiallelic recessive Bymovirus resistance in Hordeum vulgare (L.). Plant J 42:912–922

    Article  PubMed  CAS  Google Scholar 

  • Takahashi H, Suzuki M, Natsuaki K, Shigyo T, Hino K, Teraoka T, Hosokawa D, Ehara Y (2001) Mapping the virus and host genes involved in the resistance response in Cucumber mosaic virus-infected Arabidopsis thaliana. Plant Cell Physiol 42:340–347

    Article  PubMed  CAS  Google Scholar 

  • Valkonen JP, Watanabe KN (1999) Autonomous cell death, temperature sensitivity and the genetic control associated with resistance to Cucumber mosaic virus (CMV) in diploid potatoes (Solanum spp). Theor Appl Genet 99:996–1005

    Article  Google Scholar 

  • van Leeuwen H, Monfort A, Zhang HB, Puigdomenech P (2003) Identification and characterisation of a melon genomic region containing a resistance gene cluster from a constructed BAC library. Microcolinearity between Cucumis melo and Arabidopsis thaliana. Plant Mol Biol 51:703–718

    Article  PubMed  Google Scholar 

  • Voinnet O (2001) RNA silencing as a plant immune system against viruses. Trends Genet 17:449–459

    Article  PubMed  CAS  Google Scholar 

  • Yoshii M, Yoshioka N, Ishikawa M, Naito S (1998) Isolation of an Arabidopsis thaliana mutant in which the multiplication of both Cucumber mosaic virus and Turnip Crinkle virus is affected. J Virol 72:8731–8737

    PubMed  CAS  Google Scholar 

  • Yoshii M, Nishikiori M, Tomita K, Yoshioka N, Kozuka R, Naito S, Ishikawa M (2004) The Arabidopsis cucumovirus multiplication 1 and 2 loci encode translation initiation factors 4E and 4G. J Virol 78:6102–6111

    Article  PubMed  CAS  Google Scholar 

  • Zamir D (2001) Improving plant breeding with exotic genetic libraries. Nat Rev Genet 2:983–989

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

We thank Antonio Ortigosa and Fuensanta García for technical assistance, José Aramburu for his technical help with CMV infections, Juan José Lopez-Moya for CMVCP PCR primers, Catherine Dogimont for providing P9 and TL CMV strains and Marisol Luis-Arteaga for providing P104.82 strain. The work was funded by grant AGL2006-12780-C02-01/AGR from the Spanish Ministerio de Educación y Ciencia (MEC). A.E. was supported by an IAMZ fellowship, and J.A. D.-P. and A.M. M.-H. were supported by the Ministerio de Educación y Ciencia with a Juan de la Cierva and Ramón y Cajal contracts, respectively.

Note added in proofs

During the publishing procedure a paper concerning the mapping of markers ECM67 and ECM105 has been published. The reference is Fernández-Silva, I Moreno E, Eduardo I, Arús P, Alvarez JM, Monforte AJ. (2008) On the Genetic Control of Heterosis for Fruit Shape in Melon (Cucumis Melo L.). J. of Heredity DOI 10.1007/s00122-008-0883-3.

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  1. Antonio J. Monforte

    Present address: Instituto de Biología Molecular y Celular de Plantas (IBMCP)(CSIC-UPV) Ingeniero Fausto Elio, s/n CPI “Ciudad Politécnica de la Innovación DE LA INNOVACIÓN”- Edificio 8E, 46022, Valencia, Spain

Authors and Affiliations

  1. IRTA, Centre de Recerca en Agrigenòmica CSIC-IRTA-UAB, Carretera de Cabrils, Km 2, 08348, Cabrils, Barcelona, Spain

    Ali Essafi, Antonio J. Monforte, Jordi Garcia-Mas & Ana M. Martín-Hernández

  2. Estación Experimental “La Mayora” CSIC, 29759, Algarrobo-Costa, Málaga, Spain

    Juan A. Díaz-Pendón & Enrique Moriones

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  1. Ali Essafi
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Correspondence to Ana M. Martín-Hernández.

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Communicated by I. L. Goldman.

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Essafi, A., Díaz-Pendón, J.A., Moriones, E. et al. Dissection of the oligogenic resistance to Cucumber mosaic virus in the melon accession PI 161375. Theor Appl Genet 118, 275–284 (2009). https://doi.org/10.1007/s00122-008-0897-x

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