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Fine genetic mapping of xa24, a recessive gene for resistance against Xanthomonas oryzae pv. oryzae in rice

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Abstract

Bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo), is the most devastating plant bacterial disease worldwide. Different bacterial blight resistance (R) genes confer race-specific resistance to different strains of Xoo. We fine mapped a fully recessive gene, xa24, for bacterial blight resistance to a 71-kb DNA fragment in the long arm of rice chromosome 2 using polymerase chain reaction-based molecular markers. The xa24 gene confers disease resistance at the seedling and adult stages. It mediates resistance to at least the Philippine Xoo races 4, 6 and 10 and Chinese Xoo strains Zhe173, JL691 and KS-1-21. Sequence analysis of the DNA fragment harboring the dominant (susceptible) allele of xa24 suggests that this gene should encode a novel protein that is not homologous to any known R proteins. These results will greatly facilitate the isolation and characterization of xa24. The markers will be convenient tools for marker-assisted selection of xa24 in breeding programs.

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Acknowledgments

We thank the International Rice Research Institute for providing rice variety DV86 and Philippine strains of Xoo. This work was supported by grants from the National Program of High Technology Development of China, the National Program on the Development of Basic Research in China, and the National Natural Science Foundation of China.

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China

    Xiaoming Wu, Xianghua Li, Caiguo Xu & Shiping Wang

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  1. Xiaoming Wu
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  2. Xianghua Li
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  3. Caiguo Xu
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  4. Shiping Wang
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Corresponding author

Correspondence to Shiping Wang.

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Communicated by Y. Xu.

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Wu, X., Li, X., Xu, C. et al. Fine genetic mapping of xa24, a recessive gene for resistance against Xanthomonas oryzae pv. oryzae in rice. Theor Appl Genet 118, 185–191 (2008). https://doi.org/10.1007/s00122-008-0888-y

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  • DOI: https://doi.org/10.1007/s00122-008-0888-y

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