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CAPs markers to assist selection for low vicine and convicine contents in faba bean (Vicia faba L.)

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Abstract

The antinutritional factors (ANFs) present in Vicia spp. seeds are a major constraint to the wider utilization of these crops as grain legumes. In the case of faba bean (Vicia faba L.), a breeding priority is the absence vicine and convicine (v-c); responsible for favism in humans and for the reduced animal performance or low egg production in laying hens. The discovery of a spontaneous mutant allele named vc-, which induces a 10–20 fold reduction of v-c contents, may facilitate the process. However, the high cost and difficulty of the chemical detection of v-c seriously restricts the advances in breeding-selection. To identify random amplified polymorphic DNA (RAPD) markers linked to this gene, we have analysed an F2 population derived from a cross between a line with high v-c content (Vf6) and the vc- genotype (line 1268). Quantification of v-c was done by spectrophotometry on the parents and the F2 population (n = 136). By using bulked segregant analysis (BSA), two RAPD markers linked in coupling and repulsion phase to the allele vc- were identified and further converted into sequence characterized amplified regions (SCARs). Amplification of SCARS was more consistent, although the initial polymorphism between pools was lost. To recover the polymorphisms several approaches were explored. Restriction digestion with HhaI (for SCAR SCH01620) and RsaI (for SCAR SCAB12850) revealed clear differences between the parental lines. The simultaneous use of the two cleavage amplified polymorphism (CAP) markers will allow the correct fingerprinting of faba bean plants and can be efficiently used in breeding selection to track the introgression of the vc- allele to develop cultivars with low v-c content and improved nutritional value.

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Acknowledgments

This study was funded by the grants PETRI 95–0682-OP from the Spanish Science and Technology Ministry and by the European Community project EUFABA QLK5-CT2002-02307. The authors thank B. Raffiot, J. Prieto and G. de Lara for their excellent technical assistance. N. Gutierrez acknowledges a fellowship from the Consejería de Innovación Ciencia y Empresa from the Junta de Andalucía. C.M. Avila acknowledges financial support from the Spanish Ministerio de Educación y Ciencia (“Juan de la Cierva program”).

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Authors and Affiliations

  1. Instituto de Agricultura Sostenible-CSIC, Apdo. 4084, 14080, Córdoba, Spain

    N. Gutierrez & M. J. Suso

  2. Departamento de Genética, ETSIAM-UCO, Edificio Mendel (2° Planta), Campus de Rabanales, 14071, Córdoba, Spain

    C. M. Avila

  3. INRA, URLEG, BP 86510, 21065, Dijon Cedex, France

    G. Duc & P. Marget

  4. Area de Mejora y Biotecnología, CIFA “Alameda del Obispo”, IFAPA-CICE, Junta de Andalucía, Apdo. 3092, 14080, Córdoba, Spain

    M. T. Moreno & A. M. Torres

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  1. N. Gutierrez
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  2. C. M. Avila
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  3. G. Duc
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  4. P. Marget
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  5. M. J. Suso
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  6. M. T. Moreno
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  7. A. M. Torres
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Correspondence to N. Gutierrez.

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Communicated by D.A. Hoisington.

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Gutierrez, N., Avila, C.M., Duc, G. et al. CAPs markers to assist selection for low vicine and convicine contents in faba bean (Vicia faba L.). Theor Appl Genet 114, 59–66 (2006). https://doi.org/10.1007/s00122-006-0410-3

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  • DOI: https://doi.org/10.1007/s00122-006-0410-3

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