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Molecular mapping of a novel gene, Grh5, conferring resistance to green rice leafhopper (Nephotettix cincticeps Uhler) in rice, Oryza sativa L.

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Abstract

The green rice leafhopper (GRH), Nephotettix cincticeps Uhler, is one of the most serious insect pests affecting cultivated rice (Oryza sativa L.) in temperate regions of East Asia. An accession of the wild rice species, Oryza rufipogon Griff. (W1962), was found to be highly resistant to GRH by an antibiosis test. To understand the genetic basis of the GRH resistance, a BC1F1 population derived from a cross between a susceptible Japonica variety, Taichung 65 (T65), and a highly resistant accession W1962 was analyzed by quantitative trait loci (QTL) mapping. A single major QTL for GRH resistance was detected on rice chromosome 8. A nearly isogenic population containing segments of the targeted QTL region derived from W1962 was then developed through advanced backcrossing with marker-assisted selection. Further molecular mapping using a BC4F2 population revealed that a new resistance gene, designated as Green rice leafhopper resistance 5 (Grh5), was located on the distal region of the long arm of chromosome 8 and tightly linked to the simple sequence repeat markers RM3754 and RM3761. A nearly isogenic line (NIL) carrying Grh5 was subsequently developed in the progeny of the mapping population. The resistance level of Grh5-NIL was compared with those of developed NILs for GRH resistance and was found to have the highest resistance. The DNA markers found to be closely linked to Grh5 would be useful for marker-assisted selection for the improvement of resistance to GRH in rice.

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Acknowledgements

We are grateful to Dr. M. D. McMullen for critical comments and suggestions. We sincerely thank Professor Jiro Chikushi, Director of Biotron Institute, Kyushu University for use of the Entomotron in the Biotron Institute.

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Authors and Affiliations

  1. Plant Breeding Laboratory, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan

    Daisuke Fujita, Kazuyuki Doi, Atsushi Yoshimura & Hideshi Yasui

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  1. Daisuke Fujita
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  2. Kazuyuki Doi
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  3. Atsushi Yoshimura
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  4. Hideshi Yasui
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Correspondence to Hideshi Yasui.

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Communicated by M. Bohn

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Fujita, D., Doi, K., Yoshimura, A. et al. Molecular mapping of a novel gene, Grh5, conferring resistance to green rice leafhopper (Nephotettix cincticeps Uhler) in rice, Oryza sativa L.. Theor Appl Genet 113, 567–573 (2006). https://doi.org/10.1007/s00122-006-0270-x

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  • DOI: https://doi.org/10.1007/s00122-006-0270-x

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