Abstract
Skeletal myogenesis is a multistep process starting with progenitor cell proliferation, followed by their exit from the cell cycle, differentiation, alignment, and fusion to form multinucleated myotubes, typical of the differentiated muscle tissue. While the molecular players involved in early myogenesis have been extensively characterized, information about the later steps of the process is scanty. Here, we describe a novel myogenic cell line (MYOP7), composed of highly proliferating Sca-1+ muscle precursor cells, which can be induced to terminally differentiate into spontaneously contracting multinucleated myotubes. By performing high-density microarray analysis on these cells, we identified a series of genes, differentially expressed in proliferating vs differentiating conditions, which are candidates to play a major role in the later phase of myogenesis. In addition, we confirmed that the late stages of muscle differentiation are characterized by a marked upregulation of the cellular receptors for the vascular endothelial growth factor.
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Acknowledgments
This work was supported by grants from the FIRB program of the “Ministero dell’Istruzione, Universita’ e Ricerca”, Italy, from the “Fondazione CRTrieste” of Trieste, Italy and from Regione FVG, Italy.
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Mauro Giacca and Srdjan Djurovic share senior authorship.
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Supplementary Figure
Differential expression of genes involved in proliferation and differentiation pathway in proliferating and differentiated MYOP7 cells. a Differential hybridization of 136 genes annotated as “Cell proliferation” (GO:0008283) displaying statistically significant altered expression in proliferating as compared to differentiated MYOP7 cells. b Hybridization of 231 genes defined as “Cell differentiation” (GO:0030254), with about 70 genes showing differential expression between proliferating and differentiated MYOP7 cells. c Hybdridization of genes annotated with “Development”, n = 799 (GO:0007275). In all panels, D Differentiation, P proliferation (PDF 884 kb)
Supplementary Fig. 1
Differential expression of genes involved in wound healing and of genes belonging to the Notch pathway. a Differential hybridization of genes annotated as “Response to wounding” (GO: 0009611; n = 89), displaying altered expression in proliferating compared to differentiated MYOP7 cells. b Differential expression of genes belonging to the Notch pathway (PDF 447 kb)
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Zacchigna, S., Østli, E.K., Arsic, N. et al. A novel myogenic cell line with phenotypic properties of muscle progenitors. J Mol Med 86, 105–115 (2008). https://doi.org/10.1007/s00109-007-0268-0
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DOI: https://doi.org/10.1007/s00109-007-0268-0