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Cloning and characterization of a novel cardiac-specific kinase that interacts specifically with cardiac troponin I

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Abstract

Cardiac-restricted genes play important roles in cardiovascular system. In an effort to identify such novel genes we identified a novel cardiac-specific kinase gene TNNI3K localized on 1p31.1 based on bioinformatics analyses. Sequence analysis suggested that TNNI3K is a distant family member of integrin-linked kinase. Northern blot and 76-tissue array analyses showed that TNNI3K is highly expressed in heart, but is undetectable in other tissues. Immunohistochemical analysis predominantly localized TNNI3K in the nucleus of cardiac myocytes. In vitro kinase assay showed that TNNI3K is a functional kinase. The yeast two-hybrid system showed that TNNI3K could directly interact with cardiac troponin I, results that were further confirmed by coimmunoprecipitation in vivo. Our data suggest that TNNI3K is a cardiac-specific kinase and play important roles in cardiac system.

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Abbreviations

Ab :

Antibody

cTnI :

Cardiac troponin I

ILK :

Integrin-linked kinase

SDS:

Sodium dodecyl sulfate

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Acknowledgements

We are grateful to Prof. Jian Tang's assistance in generation of polyclonal antibodies against TNNI3K and Prof. Boqin Olang and Prof. Minshun Zhao's help in many respects. This work was supported by the National Natural Science Foundation of China (no. 30070313) and National 973 Project of China (no. G2000056901).

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Authors and Affiliations

  1. 167 Beilishi Road, West District, 100037, Beijing, P.R. China

    Jin-Feng Ding

  2. Molecular Medicine Center for Cardiovascular Diseases, Cardiovascular Institute and Fu Wai Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, P.R. China

    Yong Zhao, Xian-Min Meng, Ying-Jie Wei, Xiu-Wen Zhao, Dong-Qing Liu, Hui-Qing Cao & Jin-Feng Ding

  3. Cardiovascular Genome Unit, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA

    Choong-Chin Liew

Authors
  1. Yong Zhao
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  2. Xian-Min Meng
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  3. Ying-Jie Wei
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  4. Xiu-Wen Zhao
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  5. Dong-Qing Liu
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  7. Choong-Chin Liew
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  8. Jin-Feng Ding
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Corresponding author

Correspondence to Jin-Feng Ding.

Additional information

The nucleotide sequence reported here has been deposited in the GenBank database under GenBank accession number AF116826. The gene symbol TNNI3K has been approved by the HUGO Gene Nomenclature Committee

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Zhao, Y., Meng, XM., Wei, YJ. et al. Cloning and characterization of a novel cardiac-specific kinase that interacts specifically with cardiac troponin I. J Mol Med 81, 297–304 (2003). https://doi.org/10.1007/s00109-003-0427-x

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  • DOI: https://doi.org/10.1007/s00109-003-0427-x

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