Abstract.
Objective: The action of antibacterial cathelicidin CAP11 (cationic antibacterial polypeptide of 11 kDa) on the lipopolysaccharide (LPS)-induced suppression of neutrophil apoptosis was evaluated in vitro.
Methods: Human neutrophils (106 cells/ml) were incubated alone or with mononuclear cells (6 × 105 cells/ml) in the presence of LPS (10 ng/ml) and CAP11 (0.1 ~ 10 μg/ml), and neutrophil apoptosis was determined.
Results: LPS suppressed neutrophil apoptosis, accompanied with the activation of NF-κB, phosphorylation of extracellular signal-related protein kinase (ERK), expression of Bcl-XL (an anti-apoptotic protein) and inhibition of caspase 3 activity. Interestingly, CAP11 (>1 μg/ml) reversed the actions of LPS to trigger these changes, and induced neutrophil apoptosis (p < 0.0001). Moreover, neutralizing antibodies against Mac-1 (CD11b/CD18) and Toll-like receptor (TLR) 4 completely blocked the LPS-induced suppression of neutrophil apoptosis (p < 0.0001), suggesting a major role of Mac-1 and TLR4 in the LPS-mediated neutrophil activation. In addition, LPS activated monocytes to produce proinflammatory cytokines (IL-1β, TNF-α and IL-8) and inhibited neutrophil apoptosis. Importantly, CAP11 (>1 μg/ml) reduced the cytokine production, thereby inducing neutrophil apoptosis (p < 0.0001). Finally, CAP11 (>1 μg/ml) strongly suppressed the LPS-binding to neutrophils and monocytes (p < 0.01).
Conclusions: CAP11 is able to block the LPS-induced survival of neutrophils via the suppression of anti-apoptotic signaling in neutrophils and cytokine production from monocytes by inhibiting the binding of LPS to target cells.
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Received 21 April 2004; returned for revision 10 June 2004; accepted by M. Katori 14 June 2004
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Nagaoka, I., Yomogida, S., Tamura, H. et al. Antibacterial cathelicidin peptide CAP11 inhibits the lipopolysaccharide (LPS)-induced suppression of neutrophil apoptosis by blocking the binding of LPS to target cells. Inflamm. res. 53, 609–622 (2004). https://doi.org/10.1007/s00011-004-1300-2
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DOI: https://doi.org/10.1007/s00011-004-1300-2