Abstract
The presence ofClavibacter michiganensis subsp.sepedonicus was detected by immunofluorescence and ELISA in diluent in which cores from 200 potato tubers had been incubated on a rotary shaker. By this procedure the ring rot pathogen was detected in composite samples from naturally infected tubers and tuber samples “spiked” with infected tissue or a pure culture of the pathogen. Populations of saprophytic bacteria increased about 100-fold in the diluent during 18 h of incubation but did not interfere with serological detection of C.m. sepedonicus. Some of the results suggest thatC. m. sepedonicus cells multiply during incubation of tuber samples. Extraction of ring rot bacteria from potato tuber tissue by incubation on a rotary shaker requires little work and minimizes the amount of plant tissue in the extract compared to the standard extraction procedure involving maceration and differential centrifugation.
Compendio
Se detectó la presencia deClavibacter michiganensis subsp.sepedonicus por inmunofluorescencia y ELISA, en una dilución en la cual las partes centrales de 200 tubérculos de papa habían sido incubadas sobre un agitador giratorio. Siguiendo este procedimiento se detectó al agente de la pudrición en anillo en una mezcla de muestras de tubérculos infectados naturalmente y de muestras de tubérculos “pinchados” con tejido infectado o con un cultivo puro del patógeno. Las poblaciones de bacterias soprofíticas se incrementaron en la dilución aproximadamente en 100 veces durante 18 horas de incubación, pero no interfirieron con la detección serológica de C.michiganensis subsp.sepedonicus. Algunos de los resultados sugieren que las células de C.m. sepedonicus se multiplican durante la incubación de las muestras de tubérculos. La extracción de las bacterias de la pudrición en anillo de los tejidos de los tubérculos de papa por incubación sobre un agitador giratorio requiere poco trabajo y minimiza la cantidad de tejido vegetal en el extracto en comparación con el procedimiento estóndar de extracción que incluye maceración y centrifugación diferencial.
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Dinesen, I.G., De Boer, S.H. Extraction ofclaviba cter michiganensis subsp.sepedonicus from composite samples of potato tubers. American Potato Journal 72, 133–142 (1995). https://doi.org/10.1007/BF02987285
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DOI: https://doi.org/10.1007/BF02987285