Abstract
Purpose: A model for differentiating live and dead sperm cells during intracytoplasmic sperm injection (ICSI) is proposed.
Methods: We used pentoxifylline, a phosphodiesterase inhibitor known to enhance sperm motility, to initiate motility in testicular spermatozoa. Ten immotile testicular sperm samples were divided into two parts for examination of sperm motility with and without pentoxifylline treatment at 30, 60, and 90 min.
Results: The samples without pentoxifylline remained immotile even after 90 min of incubation: the addition of pentoxifylline initiated sperm motility in all samples: 51.8±10.2, 64.4±9.4, and 70.8±8.9% (mean±SD) at 30, 60, and 90 min, respectively.
Conclusions: That pentoxifylline may be used to differentiate live testicular spermatozoa during ICSI, which may improve fertilization and pregnancy rates, is suggested.
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References
Schoysman R, Vanderzwalmen P, Nijs M, Segal L, Segal-Bertin G, Geerts L, van Roosendaal E, Schoysman D: Pregnancy after fertilization with human testicular spermatozoa. Lancet 1993;342:1237
Devroey P, Liu J, Nagy Z, Goossens A, Tournaye H, Camus M, Van Steirteghem, Silber S: Pregnancies after testicular sperm extraction and intracytoplasmic sperm injection in non-obstructive azoospermia. Hum Reprod 1995;10:1457–1460
Hendry WE, Cole PJ: Fertility in men with primary ciliary dyskinesia presenting with respiratory infection. Thorax 1994;49:684–768
Tournaye H, Liu J, Nagy PZ, Camus M, Goossens A, Silber S, VanSteirteghem AC, Devroey P: Correlation between testicular histology and outcome after intracytoplasmic sperm injection using testicular spermatozoa. Hum Reprod 1996;11:127–132
Tournaye H, Liu J, Nagy Z, Verheyen G, Van Steirteghem A, Devroey P: The use of testicular sperm for intracytoplasmic sperm injection in patients with necrozoospermia. Fertil Steril 1996;66:331–334
Tasdemir M, Tasdemir I, Kodama H, Tanaka T: Pentoxifylline-enhanced acrosome reaction correlates with fertilization in vitro. Hum Reprod 1993;8:2102–2107
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Taşdemir, I., Taşdemir, M. & Tavukçuoǧlu, Ş. Effect of pentoxifylline on immotile testicular spermatozoa. J Assist Reprod Genet 15, 90–92 (1998). https://doi.org/10.1007/BF02766832
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DOI: https://doi.org/10.1007/BF02766832