Abstract
Fed-batch cultures of recombinantEscherichia coli strains were carried out for the production of poly(3-hydroxybutyric acid) (PHB) in a chemically defined medium. TheE. coli strains used were XL1-Blue, harboring pSYL105, a stable high-copy number plasmid containing theAlcaligenes eutrophus polyhydroxyalkanoate (PHA) genes, and XL1-Blue, harboring pSYL107, which is pSYL105 containing theE. coli ftsZ gene to suppress filamentation. With XL1-Blue(pSYL105) the final cell mass and PHB concentration obtained in 62 h were 102 and 22.5 g/L, respectively. Fed-batch culture of XL1-Blue(pSYL107) under identical conditions resulted in a final cell mass and PHB concentration of 127.5 and 48.2 g/L, respectively. The PHB contents obtained with XL1-Blue(pSYL105) and XL1-Blue(pSYL107) were 22.1 and 37.8%, respectively. Therefore, PHB was more efficiently produced in a defined medium by employing filamentation-suppressed recombinantE. coli.
References
A. J. Anderson and E. A. Dawes (1990)Microbiol. Rev. 54, 450–472.
Y. Poirier, C. Nawrath, and C. Somerville (1995)Bio/Technology 13, 142–150.
S. Y. Lee (1996)Biotechnol. Bioeng. 49, 1–14.
S. Y. Lee and H. N. Chang (1995)Adv. Biochem. Eng. Biotechnol. 52, 27–58.
O. P. Peoples and A. J. Sinskey (1989)J. Biol. Chem. 264, 15298–15303.
P. Schubert, A. Steinbuchel, and H. G. Schlegel (1998)J. Bacteriol. 170, 5837–5847.
S. C. Slater, W. H. Voige, and D. E. Dennis (1988)J. Bacteriol. 170, 4431–4436.
S. Y. Lee and H. N. Chang (1995)Can. J. Microbiol. 41 (Suppl. 1), 207–215.
S. Y. Lee, K. S. Yim, H. N. Chang, and Y. K. Chang (1994)J. Biotechnol. 32, 203–211.
S. Y. Lee, H. N. Chang, and Y. K. Chang (1994)Ann. N.Y. Acad. Sci. 721, 43–53.
S. Y. Lee and H. N. Chang (1994)J. Environ. Polym. Degrad. 2, 169–176.
S. Y. Lee, K. M. Lee, H. N. Chang, and A. Steinbuchel (1994)Biotechnol. Bioeng. 44, 1337–1347.
J. Lutkenhaus (1993)Curr. Opin. Genet. Dev. 3, 783–788.
S. Y. Lee (1994)Biotechnol. Lett. 16, 1247–1252.
W. J. Dower, J. F. Miller, and C. W. Ragsdale (1988)Nucleic Acids Res. 16, 6127–6145.
S. Y. Lee and H. N. Chang (1993)Biotechnol. Lett. 15, 971–974.
G. Braunegg, B. Sonnleitner, and R. M. Lafferty (1978)Eur. J. Appl. Microbiol. Biotechnol. 6, 29–37.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lee, Y., Lee, S.Y. Enhanced production of poly(3-hydroxybutyrate) by filamentation-suppressed recombinantEscherichia coli in a defined medium. J Environ Polym Degr 4, 131–134 (1996). https://doi.org/10.1007/BF02074874
Issue Date:
DOI: https://doi.org/10.1007/BF02074874