Skip to main content
SpringerLink
Log in

Autoantibodies in systemic lupus erythematosus and normal subjects

  • Originals
  • Published:
Clinical Rheumatology Aims and scope Submit manuscript

Summary

The presence of various antibodies in serum samples from patients with systemic lupus erythematosus (SLE) and from healthy subjects was investigated by ELISA, using a panel of natural antigens. Fifty-eight serum samples from 58 healthy women and 50 serum samples from 30 patients with active SLE were tested with 9 natural antigens (ds-DNA, actin, tubulin, thyroglobulin, myosin, myoglobin, human transferrin, human interferon a and BSA FV). It was found that the proportion of positive sera from healthy women at a dilution of 1/20 was almost the same as that of lupus sera at a dilution of 1/150 for nearly all antigens, while at a dilution of 1/150 the proportion of positive sera from patients with SLE was significantly higher for nearly all antigens. In lupus sera a high degree of correlation was observed between titers of anti-DNA and titers of the other antibodies. One hundred eighty-eight serum samples from 53 SLE patients, taken during exacerbation and remission of the disease were tested with ds-DNA, actin and tubulin. Antibodies (IgG) to ds-DNA actin and tubulin were found in the majority of serum samples taken during the active phase of the disease. On the other hand, very few serum samples taken during remission were found to be positive. A high degree of correlation was found between the OD of anti-actin/anti-ds-DNA (r=0.769) and anti-tubulin/anti-ds-DNA (r=0.829). In a competitive enzyme immunoassay for DNA, actin, tubulin, myosin and thyroglobulin, a high degree of inhibition was observed with the homologous antigens. Cross inhibition was observed between actin, tubulin and myosin, and to a lesser degree with DNA. These results indicate that normal sera contain low titers of auto and foreign antibodies while active SLE sera react strongly with the same antigens.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Koffler, D., Carr, R., Agnello, V., Thoburn, R., Kunkel, H.G. Antibodies to polynucleotides in human sera: antigenic specificity and relation to disease. J Exp Med 1971, 134, 294–312.

    PubMed  CAS  Google Scholar 

  2. Schleider, M.A., Nachman, R.L., Jaffe, E.A., Coleman, M.A. Clinical study of the lupus anticoagulant. Blood 1976, 48, 499–509.

    PubMed  CAS  Google Scholar 

  3. Schoenfeld, Y., Spaulian, E., Shaklai, M., Kruglack, J., Feverman, E., Pinkhas, J. Circulating anticoagulant and serological tests for syphilis. Acta Dermatol Venereol (Stockh) 1980, 60, 365–367.

    Google Scholar 

  4. Panem, S., Check, I.J., Henriksen, D., Vilcek, J. Antibodies to a-interferon in a patient with systemic lupus erythematosus. J Immunol 1982, 129, 1–3.

    PubMed  CAS  Google Scholar 

  5. Scwartz, R. Monoclonal lupus autoantibodies. Immunology Today. 1983, 4, 68–69.

    Google Scholar 

  6. Datta, S.K., Stollar, B.D., Schwartz, R.S. Normal mice express idiotypes related to autoantibody idiotypes of lupus mice. Proc Natl Acad Sci USA 1983, 80, 2723–2727.

    PubMed  CAS  Google Scholar 

  7. Primi, D., Smith, C.I.E., Hammarström, L., Möller, G. Polyclonal B-cell activators induce immunological response to autologous serum proteins. Cell Immunol 1977, 34, 367–375.

    PubMed  CAS  Google Scholar 

  8. Shoenfeld, Y., Schwartz, R. Immunological and genetic factors in autoimmune diseases. New Engl J Med 1984, 311, 1019–1029.

    Article  PubMed  CAS  Google Scholar 

  9. Avrameas, S., Guilbert, B., Dighiero, G. Natural antibodies against tubulin, actin, myoglobin, thyroglobulin, fetuin, albumin and transferrin are present in normal human sera and monoclonal immunoglobulins from multiple myeloma and Waldenstrom macroglobulinemia may express similar antibody specificities. Ann Immunol (Inst Pasteur) 1981, 132, C, 231–236.

    Google Scholar 

  10. Guilbert, B., Dighiero, G., Avrameas, S. Naturally occurring antibodies against nine common antigens in human sera. I. Detection, isolation, and characterization. J Immunol 1982, 128, 2779–2787.

    PubMed  CAS  Google Scholar 

  11. Shoenfeld, Y., Rauch, J., Massicotte, H., Datta, S.K., Andre Schwartz, J., Stollar, B.D., Schwartz, R.S. Polyspecificity of monoclonal lupus autoantibodies produced by human-human hybridomas. New Engl J Med 1983, 308, 414–420.

    Article  PubMed  CAS  Google Scholar 

  12. Solomon, G., Schiffenbauer, J., Keiser, H.D., Diamond, B. Use of monoclonal antibodies to identify shared idiotypes on human antibodies to native DNA from patients with systemic lupus erythematosus. Proc Natl Acad Sci USA 1983, 80, 850–854.

    PubMed  CAS  Google Scholar 

  13. Larrick, J.W., Hyman, E.S. Acquired iron-deficiency anemia caused by an antibody against the transferrin receptor. New Engl J Med 1984, 311, 214–218.

    Article  PubMed  CAS  Google Scholar 

  14. Rousset, B., Bernier-Valentin, F., Poncet, C., Orgiazzi, J., Madec, A.M., Monier, J.C., Mornex, R. Anti-tubulin antibodies in autoimmune thyroid disorders. Clin Exp Immunol 1983, 52, 325–332.

    PubMed  CAS  Google Scholar 

  15. Pateraki, E., Portocala, R., Labrousse, H., Guesdon, J.L. Antiactin and antitubulin antibodies in canine visceral leishmaniasis. Infect Immun 1983, 42, 496–500.

    PubMed  CAS  Google Scholar 

  16. Suit, B.E., Axelrod, D., Moutsopoulos, H.P., Decker, J.L., Hooks, J.J. Detection of anti-interferon antibodies in systemic lupus erythematosus. Clin Exp Rheum 1983, 1, 133–135.

    CAS  Google Scholar 

  17. Wallis, P.J.W., Clark, C.J.M. Visceral leishmaniasis complicating systemic lupus erythematosus. Ann Rheum Dis 1983, 42, 201–202.

    Article  PubMed  CAS  Google Scholar 

  18. Tan, E.M., Cohen, A.S., Fries, J.F., Masi, A.T., McShane, D.J., Rothfield, N.F., Schaller, J.G., Talal, N., Winchester, R.J. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 1982, 25, 1271–1277.

    PubMed  CAS  Google Scholar 

  19. Rivero, S.J., Llorente, L., Diaz-Jouanen, E., Alarcon-Segovia, D. T-lymphocyte subpopulation in untreated SLE variations with disease activity. Arthritis Rheum 1977, 20, 1169–1173.

    PubMed  CAS  Google Scholar 

  20. Martin, W.J., Martin, S.E. Thymus reactive IgM auto-antibodies in normal mouse sera. Nature 1975, 254, 716–718.

    PubMed  CAS  Google Scholar 

  21. Sela, B.A., Wang, J.L., Edelman, G.M. Antibodies reactive with cell surface carbohydrates. Proc Natl Acad Sci 1975, 72, 1127–1131.

    PubMed  CAS  Google Scholar 

  22. Karsenti, E., Guilbert, B., Bornens, M., Avrameas, S. Antibodies to tubulin in normal nonimmunized animals. Proc Natl Acad Sci 1977, 74, 3997–4001.

    PubMed  CAS  Google Scholar 

  23. Martin, S.E., Martin, W.J. Interspecies brain antigen detected by naturally occurring mouse antibrain auto-antibody. Proc Natl Acad Sci 1975, 72, 1036–1040.

    PubMed  CAS  Google Scholar 

  24. Walford, R.L. The immunologic theory of aging: Munksgaard Copenhagen 1969.

  25. Dighiero, G., Lymberi, P., Mazie, J.C., Rouyres Butler-Browne, G.S., Whalen, R.G., Avrameas, S. Murine hybridomas secreting natural monoclonal antibodies reacting with self antigens. J Immunol 1983, 131, 2267–2272.

    PubMed  CAS  Google Scholar 

  26. Cunningham, A.J. Self-tolerance maintained by active suppressor mechanisms. Transplant Rev 1976, 31, 23–43.

    PubMed  CAS  Google Scholar 

  27. Madaio, M.P., Hodder, S., Schwartz, R.S., Stollar, B.D. Responsiveness of autoimmune and normal mice to nucleic acic antigens. J Immunol 1984, 132, 872–876.

    PubMed  CAS  Google Scholar 

  28. Theophilopoulos, A.N., Eisenberg, R.A., Bourdon, M., Crowell, J.S.Jr., Dixon, F.J. Distribution of lymphocytes identified by surface markers in murine strains with systemic lupus erythematosus-like syndromes. J Exp Med 1979, 149, 516–534.

    Google Scholar 

  29. Miller, K.B., Schwartz, R.S. Autoimmunity and suppressor T-lymphocytes. Adv Intern Med 1982, 27, 281–313.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut Pasteur Hellénique, University of Athens Medical School, 115 27, Athens, Greece

    E. Pateraki & R. Portocalas

  2. Department of Hygiene and Epidemiology, University of Athens Medical School, 115 27, Athens, Greece

    E. Kaklamani

  3. First Department of Internal Medicine, University of Athens Medical School, 115 27, Athens, Greece

    Ph. Kaklamanis & A. Aessopos

Authors
  1. E. Pateraki
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. Kaklamani
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ph. Kaklamanis
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. Portocalas
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. Aessopos
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pateraki, E., Kaklamani, E., Kaklamanis, P. et al. Autoantibodies in systemic lupus erythematosus and normal subjects. Clin Rheumatol 5, 338–345 (1986). https://doi.org/10.1007/BF02054252

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02054252

Key words

Search

Navigation