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Simplification of the ecdysteroid radioimmunoassay by the use of protein A fromStaphylococcus aureus

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Summary

Three methods of separating antibody-bound ligand from free ligand were compared for an ecdysteroid radioimmunoassay. Ecdysteroid antibody concentration and ligand specific radioactivity were adjusted to measure 2 ranges of ecdysone concentrations (0.01–2.0 ng and 0.25–32.0 ng). In comparison with the tradiotional separating agent, ammonium sulfate, neither protein A nor polyethylene glycol altered sensitivity or specificity of the radioimmunoassays. The protein A immunoglobulin precipitation method is quick and simple, making it a preferred alternative protocol for terminating ecdysteroid radioimmunoassays.

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Authors and Affiliations

  1. Department of Biology, University of North Carolina, Wilson Hall 046A, 27514, Chapel Hill, North Carolina, USA

    J. T. Warren, W. Smith & L. I. Gilbert

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  1. J. T. Warren
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  2. W. Smith
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  3. L. I. Gilbert
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Supported by grants AM-30118 from the National Institutes of Health and PCM-8116931 from the National Science Foundation.

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Warren, J.T., Smith, W. & Gilbert, L.I. Simplification of the ecdysteroid radioimmunoassay by the use of protein A fromStaphylococcus aureus. Experientia 40, 393–394 (1984). https://doi.org/10.1007/BF01952570

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  • DOI: https://doi.org/10.1007/BF01952570

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