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Immunochemical characterization of antibody-coated nanoparticles

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Abstract

A new method using surface plasmon resonance (SPR) through the BIAcore was used to demonstrate the specific interaction between an anti-CD4 monoclonal antibody (IOT4a), adsorbed on poly(methylidene malonate 2.1.2) (PMM 2.1.2) nanoparticles, and the CD4 molecule. The results obtained were compared with the interaction of the same immunonanoparticles with rabbit anti-mouse Fc antibodies. The molar ratio (Fc)/(Fab) was 1, suggesting that the same number of epitopes on the Fc and the Fab fragments were accessible after IOT4a adsorption onto nanoparticles. Comparing the observed association rates of free antibody and antibody adsorbed on nanoparticles, the number of molecules of IOT4a antibody on PMM 2.1.2 nanoparticles was estimated as between 2.6 and 3 per nanoparticle. The properties of the antibody-coated nanoparticles are compatible with their use as antibody-targeted pharmacophores.

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Abbreviations

rsCD4:

recombinant soluble cluster differentiation 4

HIV-1:

human immunodeficiency virus type 1

RU:

resonance unit

Np:

nanoparticles

Np-Ab:

anti-CD4 immunonanoparticles

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Authors and Affiliations

  1. Immunologie des Maladies Infectieuses, Equipe associée INRA d'Immunologie Parasitaire, UFR des Sciences Pharmaceutiques, CJF INSERM 93-09, 31 avenue Monge, F-37200, Tours, (France)

    F. Velge-Roussel, D. Bout & J. Hoebeke

  2. Laboratoires UPSA, 128 rue Danton, BP 325, F-92506, Rueil-Malmaison Cédex, (France)

    P. Breton, X. Guillon & F. Lescure

  3. VIRSOL, 46 rue Boissière, F-75116, Paris, (France)

    N. Bru

Authors
  1. F. Velge-Roussel
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  2. P. Breton
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  3. X. Guillon
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  4. F. Lescure
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  5. N. Bru
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  6. D. Bout
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  7. J. Hoebeke
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Velge-Roussel, F., Breton, P., Guillon, X. et al. Immunochemical characterization of antibody-coated nanoparticles. Experientia 52, 803–806 (1996). https://doi.org/10.1007/BF01923993

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  • DOI: https://doi.org/10.1007/BF01923993

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