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Ecdysone 20-hydroxylase from the midgut of the tobacco hornworm (Manduca sexta L.)

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Summary

An ecdysone 20-hydroxylase enzyme system that converts α-ecdysone to 20-hydroxyecdysone was prepared from the midgut of the tobacco hornworm prepupa. This partially purified enzyme is NADPH dependent and is localized in the mitochondrial fraction of the midgut tissue.

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References

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Author notes

  1. H. N. Nigg (NRC-ARS Postdoctoral Associate)

    Present address: Agricultural Research and Education Center, IEAS, University of Florida, P. O. Box 1088, 33850, Lake Alfred, Florida, USA

Authors and Affiliations

  1. Insect Physiology Laboratory, Plant Protection Institute, Agricultural Research Service, U.S. Department of Agriculture, 20705, Beltsville, Maryland, USA

    H. N. Nigg (NRC-ARS Postdoctoral Associate), J. A. Svoboda, M. J. Thompson, S. R. Dutky, J. N. Kaplanis & W. E. Robbins

Authors
  1. H. N. Nigg
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  2. J. A. Svoboda
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  3. M. J. Thompson
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  4. S. R. Dutky
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  5. J. N. Kaplanis
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  6. W. E. Robbins
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Nigg, H.N., Svoboda, J.A., Thompson, M.J. et al. Ecdysone 20-hydroxylase from the midgut of the tobacco hornworm (Manduca sexta L.). Experientia 32, 438–439 (1976). https://doi.org/10.1007/BF01920781

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  • DOI: https://doi.org/10.1007/BF01920781

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