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Transforming growth factor β gene maps to human chromosome 19 long arm and to mouse chromosome 7

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Somatic Cell and Molecular Genetics

Abstract

Transforming growth factors (TGF) are defined as biologically active polypeptides which reversibly confer the transformed phenotype onto untransformed cultured cells. They have been subdivided into two classes: type α and type β TGFs. TGF-β acts synergistically with TGF-α in inducing phenotypic transformation. TGF-β can also act as negative autocrine growth factor. A human 1050-bp EcoRI cDNA fragment was used to map the human locus for TGF-β by Southern blotting of DNA prepared from 17 human × Chinese hamster somatic cell hybrids. The humanspecific restriction fragments segregated with human chromosome 19 in all of 14 informative hybrids. All other human chromosomes were discordant with the TGF-β bands in at least four hybrids. After in situ hybridization of the tritiated TGF-β probe to normal human metaphase spreads, 151 silver grains were scored in 54 cells. Of 24 grains over chromosome 19, 16 grains (11%) lay over region 19q13.1 → q13.3. Of the 54 cells analyzed, 16 (30%) had label over region 19q13.1 → q13.3. Thus,TGFB is assigned to chromosome 19, subbands q13.1 → q13.3. TheTgf- β locus in the mouse was mapped to chromosome 7 by hybridizing a murine cDNA probe to a Chinese hamster × mouse hybrid panel. Human chromosome 19 and proximal mouse chromosome 7 share another four homologous loci.

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Author notes

  1. Jane E. Brissenden

    Present address: Department of Cellular/Structural Biology, University of Texas Health Sciences Center, 78284, San Antonio, Texas

Authors and Affiliations

  1. Department of Human Genetics, Yale University School of Medicine, 333 Cedar Street, P.O. Box 3333, 06510, New Haven, Connecticut

    David Fujii, Jane E. Brissenden & Uta Francke

  2. Department of Molecular Biology, Genentech, Inc., 460 Point San Bruno Boulevard, 94080, South San Francisco, California

    Rik Derynck

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  1. David Fujii
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  2. Jane E. Brissenden
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  3. Rik Derynck
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  4. Uta Francke
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Fujii, D., Brissenden, J.E., Derynck, R. et al. Transforming growth factor β gene maps to human chromosome 19 long arm and to mouse chromosome 7. Somat Cell Mol Genet 12, 281–288 (1986). https://doi.org/10.1007/BF01570787

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  • DOI: https://doi.org/10.1007/BF01570787

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